New practical and theoretical approaches to the induction of morphogenesis from plant tumors in vitro using new types of plant growth regulators: towards constructive paradigms in agriculture and medicine.

Using classical or traditional plant growth regulators, calli or plant tumors have been produced in vitro and subsequently have been induced to produce buds and plantlets, a process referred to as regeneration. For many years, this has been a successful procedure for in vitro, plant propagation. However, for a number of plant species investigators could not induce calli in vitro to produce buds. Organogenesis was still recalcitrant for various plants in 1980. New types or nonconventional growth regulators, such as methylglyoxal and ascorbic acid, were then found to overcome recalcitrant organogenesis in vitro. Their successful or effective use gave support to a theory that stressful, non-uniform cohesive force-fields, electromagnetic in nature, occurring through the application of certain chemicals, are necessary for in vitro morphogenesis from plant neoplasm or callus. Morphogenesis is seen as an adaptive accommodation to the inner stresses from such non-uniform, cohesive forces. Diverse chemicals, not considered traditional plant growth regulators would, it has been conjectured, enable the generation of such cohesive forces, in non-uniform arrays, and it has been predicted that more chemicals of this type will be discovered. A new constructive approach to agriculture and medicine, using a new plant tissue-culture model, based on new theory, has also been predicted.