口腔致病菌直弯曲杆菌推定趋化蛋白CheY的鉴定和特性研究。

Michael J LaGier, Ihor Bilokopytov, Bradley Cockerill, Deborah S Threadgill
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引用次数: 3

摘要

直弯曲杆菌是一种引起牙周炎的口腔细菌。有助于病原体致病能力的过程,如趋化性,在直梭菌中很大程度上是未知的。本研究的目的是为了更好地了解直肠梭菌的趋化性,通过检查直肠梭菌基因组的cheY基因的存在。CheY蛋白作为双组分反应调节因子参与趋化作用。值得注意的是,来自几种病原体(包括相关物种空肠弯曲杆菌)的CheY蛋白已被证明有助于细菌毒力。简并性PCR、RT-PCR、序列分析和结构建模表明,直肠直肠梭菌编码的基因(Cr-CheY)与先前表征的CheY蛋白具有显著的同源性。该蛋白重组形式的功能研究支持了Cr-CheY在直梭菌趋化性中的可能作用。Cr-CheY是第一个从口腔弯曲杆菌中分离到的CheY。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Identification and Characterization of a Putative Chemotaxis Protein, CheY, from the Oral Pathogen Campylobacter rectus.

Campylobacter rectus is an understudied oral bacterium that contributes to periodontitis. Processes that contribute to the disease-causing capabilities of pathogens, such as chemotaxis, are largely unknown in C. rectus. The aim of this study was to better understand C. rectus chemotaxis, by examining the C. rectus genome for the presence of a cheY gene. CheY proteins play a part in chemotaxis by acting as two-component response regulators. Significantly, CheY proteins from several pathogens, including the related species Campylobacter jejuni, have been shown to contribute to bacterial virulence. Degenerate PCR, RT-PCR, sequence analyses, and structural modeling showed that C. rectus encodes a gene (Cr-CheY) which shares significant homology with previously characterized CheY proteins. Functional studies of a recombinant form of the protein supports a likely role of Cr-CheY in C. rectus chemotaxis. Cr-CheY is the first CheY characterized from the oral campylobacters.

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