Maysa Araujo Ferreira-Julio, Marcela Souza Pinhel, Driele Cristina Gomes Quinhoneiro, Carolina Ferreira Nicoletti, Antonio Carlos Brandão, Carla Barbosa Nonino, Sidney Pinheiro, Bruno Affonso Parenti Oliveira, Michele Lima Gregório, Days Oliveira Andrade, Cristiana Cortes-Oliveira, Dorotéia Silva Souza
{"title":"肥胖人群瘦素基因LEP -2548G>A多态性及其对血脂的影响","authors":"Maysa Araujo Ferreira-Julio, Marcela Souza Pinhel, Driele Cristina Gomes Quinhoneiro, Carolina Ferreira Nicoletti, Antonio Carlos Brandão, Carla Barbosa Nonino, Sidney Pinheiro, Bruno Affonso Parenti Oliveira, Michele Lima Gregório, Days Oliveira Andrade, Cristiana Cortes-Oliveira, Dorotéia Silva Souza","doi":"10.1159/000371767","DOIUrl":null,"url":null,"abstract":"<p><strong>Background/aim: </strong>We studied the molecular pathogenesis of obesity, involving complex interactions between environmental and genetic factors, with a focus on the leptin gene. It was our aim to characterize the LEP -2548G>A leptin polymorphism and lipid profile in obese and normal-weight individuals.</p><p><strong>Methods: </strong>A total of 212 individuals were divided into the study group including 136 obese patients (body mass index, BMI≥30) and the control group with 76 normal-weight individuals (BMI>18.5 and ≤24.9). DNA was amplified by polymerase chain reaction and restriction fragment length polymorphism. The lipid profile was analyzed by enzymatic colorimetric methods. The level of significance was set at p<0.05.</p><p><strong>Results: </strong>There was a prevalence of the GA genotype in both groups. However, comparative group analysis showed an association of the recessive model (AA+GA) with increased triglycerides (TG) and decreased high-density lipoprotein cholesterol (HDL-C) levels in the study group.</p><p><strong>Conclusion: </strong>This study did not confirm an association between obesity and the LEP -2548G>A polymorphism. However, AA+GA genotypes, in the presence of obesity, seem to contribute to a reduction in HDL-C and an increase in TG compared with normal-weight individuals. This should be confirmed in further studies.</p>","PeriodicalId":54779,"journal":{"name":"Journal of Nutrigenetics and Nutrigenomics","volume":"7 4-6","pages":"225-31"},"PeriodicalIF":0.0000,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000371767","citationCount":"5","resultStr":"{\"title\":\"LEP -2548G>A Polymorphism of the Leptin Gene and Its Influence on the Lipid Profile in Obese Individuals.\",\"authors\":\"Maysa Araujo Ferreira-Julio, Marcela Souza Pinhel, Driele Cristina Gomes Quinhoneiro, Carolina Ferreira Nicoletti, Antonio Carlos Brandão, Carla Barbosa Nonino, Sidney Pinheiro, Bruno Affonso Parenti Oliveira, Michele Lima Gregório, Days Oliveira Andrade, Cristiana Cortes-Oliveira, Dorotéia Silva Souza\",\"doi\":\"10.1159/000371767\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background/aim: </strong>We studied the molecular pathogenesis of obesity, involving complex interactions between environmental and genetic factors, with a focus on the leptin gene. It was our aim to characterize the LEP -2548G>A leptin polymorphism and lipid profile in obese and normal-weight individuals.</p><p><strong>Methods: </strong>A total of 212 individuals were divided into the study group including 136 obese patients (body mass index, BMI≥30) and the control group with 76 normal-weight individuals (BMI>18.5 and ≤24.9). DNA was amplified by polymerase chain reaction and restriction fragment length polymorphism. The lipid profile was analyzed by enzymatic colorimetric methods. The level of significance was set at p<0.05.</p><p><strong>Results: </strong>There was a prevalence of the GA genotype in both groups. However, comparative group analysis showed an association of the recessive model (AA+GA) with increased triglycerides (TG) and decreased high-density lipoprotein cholesterol (HDL-C) levels in the study group.</p><p><strong>Conclusion: </strong>This study did not confirm an association between obesity and the LEP -2548G>A polymorphism. However, AA+GA genotypes, in the presence of obesity, seem to contribute to a reduction in HDL-C and an increase in TG compared with normal-weight individuals. This should be confirmed in further studies.</p>\",\"PeriodicalId\":54779,\"journal\":{\"name\":\"Journal of Nutrigenetics and Nutrigenomics\",\"volume\":\"7 4-6\",\"pages\":\"225-31\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1159/000371767\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nutrigenetics and Nutrigenomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1159/000371767\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2015/3/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutrigenetics and Nutrigenomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000371767","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2015/3/17 0:00:00","PubModel":"Epub","JCR":"Q","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
LEP -2548G>A Polymorphism of the Leptin Gene and Its Influence on the Lipid Profile in Obese Individuals.
Background/aim: We studied the molecular pathogenesis of obesity, involving complex interactions between environmental and genetic factors, with a focus on the leptin gene. It was our aim to characterize the LEP -2548G>A leptin polymorphism and lipid profile in obese and normal-weight individuals.
Methods: A total of 212 individuals were divided into the study group including 136 obese patients (body mass index, BMI≥30) and the control group with 76 normal-weight individuals (BMI>18.5 and ≤24.9). DNA was amplified by polymerase chain reaction and restriction fragment length polymorphism. The lipid profile was analyzed by enzymatic colorimetric methods. The level of significance was set at p<0.05.
Results: There was a prevalence of the GA genotype in both groups. However, comparative group analysis showed an association of the recessive model (AA+GA) with increased triglycerides (TG) and decreased high-density lipoprotein cholesterol (HDL-C) levels in the study group.
Conclusion: This study did not confirm an association between obesity and the LEP -2548G>A polymorphism. However, AA+GA genotypes, in the presence of obesity, seem to contribute to a reduction in HDL-C and an increase in TG compared with normal-weight individuals. This should be confirmed in further studies.
期刊介绍：
The emerging field of nutrigenetics and nutrigenomics is rapidly gaining importance, and this new international journal has been established to meet the needs of the investigators for a high-quality platform for their research. Endorsed by the recently founded "International Society of Nutrigenetics/Nutrigenomics", the ‘Journal of Nutrigenetics and Nutrigenomics’ welcomes contributions not only investigating the role of genetic variation in response to diet and that of nutrients in the regulation of gene expression, but is also open for articles covering all aspects of gene-environment interactions in the determination of health and disease.
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