百合病毒的纳米孔宏基因组测序快速诊断和鉴定。

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2022-10-01 DOI:10.5423/PPJ.OA.06.2022.0084
Hyo-Jeong Lee, In-Sook Cho, Rae-Dong Jeong
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引用次数: 5

摘要

百合是韩国最重要的观赏花卉作物之一。百合花的大部分病毒病是由受感染的鳞茎传播的,由于产量下降,造成严重的经济损失。各种诊断技术和高通量测序方法已被用于检测百合病毒。根据牛津纳米孔技术公司(ONT)的说法,MinION是一种紧凑的便携式测序设备。本研究利用ONT平台,在百合样品中检测到百合斑驳病毒、百合无症状病毒和车前草花叶病毒3种植物病毒。通过ONT对reads进行基因组组装,获得100%的覆盖率和90.3-93.4%的同源性。因此,我们表明ONT平台是一个很有前途的工具,用于诊断和表征感染作物的病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Nanopore Metagenomics Sequencing for Rapid Diagnosis and Characterization of Lily Viruses.

Lilies (Lilium spp.) are one of the most important ornamental flower crops grown in Korea. Most viral diseases in lilies are transmitted by infected bulbs, which cause serious economic losses due to reduced yields. Various diagnostic techniques and high-throughput sequencing methods have been used to detect lily viruses. According to Oxford Nanopore Technologies (ONT), MinION is a compact and portable sequencing device. In this study, three plant viruses, lily mottle, lily symptomless, and plantago asiatica mosaic virus, were detected in lily samples using the ONT platform. As a result of genome assembly of reads obtained through ONT, 100% coverage and 90.3-93.4% identity were obtained. Thus, we show that the ONT platform is a promising tool for the diagnosis and characterization of viruses that infect crops.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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