人类剪指甲作为基因研究的DNA来源。

Le Truong, Hannah Lui Park, Seong Sil Chang, Argyrios Ziogas, Susan L Neuhausen, Sophia S Wang, Leslie Bernstein, Hoda Anton-Culver
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引用次数: 8

摘要

传统上,血液样本被用作基因分析DNA的主要来源。然而,这种来源在收集、运输和长期储存方面可能很困难。在这项研究中,我们研究了人类指甲修剪是否可以作为SNP基因分型、零等位基因检测和全基因组扩增的DNA来源。从提取的指甲DNA中,我们获得了长达400 bp的扩增子,与来自匹配血液样本的DNA相比,SNP基因分型的一致性>96%,零等位基因检测的一致性> 100%。在全基因组扩增中,OmniPlex的SNP基因分型和零等位基因检测成功率分别为89.3%和76.8%,优于Multiple Displacement amplification。两种方法的符合率均为98%。当与OmniPlex全基因组扩增相结合时,人类指甲修剪可能被用作全血的替代品,作为一种侵入性更小、更方便的基因分型研究DNA来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Human Nail Clippings as a Source of DNA for Genetic Studies.

Blood samples have traditionally been used as the main source of DNA for genetic analysis. However, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we investigated whether human nail clippings could be used as a source of DNA for SNP genotyping, null-allele detection, and whole-genome amplification. From extracted nail DNA, we achieved amplicons up to a length of ~400 bp and >96% concordance for SNP genotyping and 100% concordance for null-allele detection compared to DNA derived from matched blood samples. For whole-genome amplification, OmniPlex performed better than Multiple Displacement Amplification with a success rate of 89.3% and 76.8% for SNP genotyping and null-allele detection, respectively. Concordance was ~98% for both methods. When combined with OmniPlex whole-genome amplification, human nail clippings could potentially be used as an alternative to whole blood as a less invasive and more convenient source of DNA for genotyping studies.

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