{"title":"从食物毒素到生物标志物:聚乙二醇化三元过渡金属硫化物在牛奶和人血清中多重检测黄曲霉毒素B1和黄曲霉毒素M1","authors":"Kanchan Yadav , Kalimuthu Moovendaran , Namasivayam Dhenadhayalan , Shang-Fan Lee , Man-Kit Leung , Raman Sankar","doi":"10.1016/j.snr.2023.100156","DOIUrl":null,"url":null,"abstract":"<div><p>Aflatoxins (AF) are fungal toxins which not only contaminate food, but also adversely impact human health if consumed and serve as biomarkers for deadly diseases like liver cancer. Therefore, there is a strong need to develop ultra-sensitive AF detection method in physiological environments. In the present study, ternary transition metal sulfides single crystals (Mn<sub>0.02</sub>Ta<sub>3</sub>S<sub>6</sub> and Fe<sub>0</sub>.<sub>65</sub>Ta<sub>3</sub>S<sub>6</sub>) were grown, exfoliated to thin layered nanosheets (NSs) followed by polyethylene-glycol modification (PEG@NSs). High fluorescence quenching abilities towards the aptamers for AF biosensing were demonstrated using PEGylated NSs. It has been observed that PEGylated NSs improves the detection sensitivity ∼ 248 times better than non-PEGylated NSs. Subsequently, multiplexed detections of aflatoxin B1 (AFB1) and M1 (AFM1) in diverse mediums including PBS buffer (1 ×), milk and human serum were performed by employing PEG@Mn<sub>0</sub>.<sub>02</sub>Ta<sub>3</sub>S<sub>6</sub> NSs with TAMRA dye-labelled AFB1 aptamer and FAM dye-labelled AFM1 aptamer, respectively. The fluorescence intensity of designed sensor exhibited ultrasensitive detections (∼ pM) and a wide linear range (≥ 5 orders). Hence, the present study can serve as a unique platform for facile, ultra-sensitive, selective, cost-effective and quick multiplexed detection of food toxins and disease biomarkers in complex-matrix.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":null,"pages":null},"PeriodicalIF":6.5000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"From food toxins to biomarkers: Multiplexed detection of aflatoxin B1 and aflatoxin M1 in milk and human serum using PEGylated ternary transition metal sulfides\",\"authors\":\"Kanchan Yadav , Kalimuthu Moovendaran , Namasivayam Dhenadhayalan , Shang-Fan Lee , Man-Kit Leung , Raman Sankar\",\"doi\":\"10.1016/j.snr.2023.100156\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Aflatoxins (AF) are fungal toxins which not only contaminate food, but also adversely impact human health if consumed and serve as biomarkers for deadly diseases like liver cancer. Therefore, there is a strong need to develop ultra-sensitive AF detection method in physiological environments. In the present study, ternary transition metal sulfides single crystals (Mn<sub>0.02</sub>Ta<sub>3</sub>S<sub>6</sub> and Fe<sub>0</sub>.<sub>65</sub>Ta<sub>3</sub>S<sub>6</sub>) were grown, exfoliated to thin layered nanosheets (NSs) followed by polyethylene-glycol modification (PEG@NSs). High fluorescence quenching abilities towards the aptamers for AF biosensing were demonstrated using PEGylated NSs. It has been observed that PEGylated NSs improves the detection sensitivity ∼ 248 times better than non-PEGylated NSs. Subsequently, multiplexed detections of aflatoxin B1 (AFB1) and M1 (AFM1) in diverse mediums including PBS buffer (1 ×), milk and human serum were performed by employing PEG@Mn<sub>0</sub>.<sub>02</sub>Ta<sub>3</sub>S<sub>6</sub> NSs with TAMRA dye-labelled AFB1 aptamer and FAM dye-labelled AFM1 aptamer, respectively. The fluorescence intensity of designed sensor exhibited ultrasensitive detections (∼ pM) and a wide linear range (≥ 5 orders). Hence, the present study can serve as a unique platform for facile, ultra-sensitive, selective, cost-effective and quick multiplexed detection of food toxins and disease biomarkers in complex-matrix.</p></div>\",\"PeriodicalId\":426,\"journal\":{\"name\":\"Sensors and Actuators Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.5000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensors and Actuators Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S266605392300019X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S266605392300019X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
From food toxins to biomarkers: Multiplexed detection of aflatoxin B1 and aflatoxin M1 in milk and human serum using PEGylated ternary transition metal sulfides
Aflatoxins (AF) are fungal toxins which not only contaminate food, but also adversely impact human health if consumed and serve as biomarkers for deadly diseases like liver cancer. Therefore, there is a strong need to develop ultra-sensitive AF detection method in physiological environments. In the present study, ternary transition metal sulfides single crystals (Mn0.02Ta3S6 and Fe0.65Ta3S6) were grown, exfoliated to thin layered nanosheets (NSs) followed by polyethylene-glycol modification (PEG@NSs). High fluorescence quenching abilities towards the aptamers for AF biosensing were demonstrated using PEGylated NSs. It has been observed that PEGylated NSs improves the detection sensitivity ∼ 248 times better than non-PEGylated NSs. Subsequently, multiplexed detections of aflatoxin B1 (AFB1) and M1 (AFM1) in diverse mediums including PBS buffer (1 ×), milk and human serum were performed by employing PEG@Mn0.02Ta3S6 NSs with TAMRA dye-labelled AFB1 aptamer and FAM dye-labelled AFM1 aptamer, respectively. The fluorescence intensity of designed sensor exhibited ultrasensitive detections (∼ pM) and a wide linear range (≥ 5 orders). Hence, the present study can serve as a unique platform for facile, ultra-sensitive, selective, cost-effective and quick multiplexed detection of food toxins and disease biomarkers in complex-matrix.
期刊介绍:
Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications.
For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.