小鼠炎症性肠病的探索:化学诱导的炎症性肠病(IBD)小鼠模型

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2017-03-02 DOI:10.1002/cpmo.20

Raffaella Maria Gadaleta, Oihane Garcia-Irigoyen, Antonio Moschetta

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引用次数: 31

摘要

炎症性肠病(IBD)是一种慢性多因素炎症性疾病，其特征是肠道炎症的激活和缓解期，具有潜在的严重并发症，可导致死亡。肠道炎症的实验动物模型对于理解克罗恩病(CD)和溃疡性结肠炎(UC)这两种主要的人类IBD表型的发病机制至关重要。动物模型有助于揭示IBD的分子背景，尽管单一模型无法捕捉这种疾病的复杂性，但每种模型都提供了对其不同方面的有价值的见解。化学诱导的肠道炎症模型，主要是葡聚糖硫酸钠(DSS)-和2,4,6-三硝基苯磺酸(TNBS)-诱导的结肠炎，被广泛使用。本文描述了DSS和tnbs诱导的结肠炎模型及其与IBD病理生理和临床前治疗管理的相关性。©2017 by John Wiley &儿子,Inc。

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Exploration of Inflammatory Bowel Disease in Mice: Chemically Induced Murine Models of Inflammatory Bowel Disease (IBD)

Inflammatory bowel disease (IBD) is a chronic multifactorial inflammatory disorder characterized by periods of activation and remission of intestinal inflammation, with potentially severe complications, that can lead to mortality. Experimental animal models of intestinal inflammation are crucial for understanding the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC), the two major human IBD phenotypes. Animal models have been instrumental in unveiling the molecular background of IBD, and although a single model is not able to capture the complexity of this disease, each of them provided valuable insight into its different aspects. Chemically induced models of intestinal inflammation, mainly dextran sodium sulfate (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis, are widely used. This article describes DSS- and TNBS-induced colitis models and their relevance to IBD pathophysiology and pre-clinical therapeutic management. © 2017 by John Wiley & Sons, Inc.

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.