小鼠眼表金黄色葡萄球菌感染模型的建立

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2017-03-02 DOI:10.1002/cpmo.23

Zhiyong Zhang, Osama Abdel-Razek, Guirong Wang

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引用次数: 3

摘要

建立一个合适的动物模型，准确地反映疾病和宿主对人类细菌感染的免疫反应，是眼表感染研究的主要挑战。几十年来，由于各种遗传背景、靶向缺陷和免疫试剂的可用性和相对较低的成本，小鼠一直是眼表感染研究的理想小动物模型。通过小鼠和细菌菌株的不同组合，小鼠感染模型可以用来探索细菌感染和眼表先天免疫的完整图景。在正常的眼部环境下，我们建立了一个金黄色葡萄球菌感染的小鼠模型，作为研究哺乳动物宿主对病原体反应的一个方便和易于处理的模型系统。©2017 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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A Mouse Model for Ocular Surface Staphylococcus aureus Infection

Creation of an appropriate animal model that accurately reflects the disease and host immune response to bacterial infection in humans is a major challenge in ocular-surface infection research. For decades, mice have been the ideal small animal model for ocular-surface infection research because of the availability and relatively low cost of various genetic backgrounds, targeted defects, and immunologic reagents. By employing different combinations of mouse and bacterial strains, murine infection models can be used to explore a complete picture of bacterial infection and innate immunity of the ocular surface. A murine model of Staphylococcus aureus infection under normal ocular circumstances is presented here as a convenient and tractable model system in which to study mammalian host responses to pathogens. © 2017 by John Wiley & Sons, Inc.

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.