光学生物传感器测量蛋白质相互作用。

Q1 Biochemistry, Genetics and Molecular Biology Current Protocols in Protein Science Pub Date : 2017-04-03 DOI:10.1002/cpps.31
Huaying Zhao, Lisa F Boyd, Peter Schuck
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引用次数: 24

摘要

本单元介绍了用于测量可逆蛋白质相互作用平衡和动力学的光学生物传感的基本技术。重点放在描述健壮的方法,将提供可靠的结果与很少的假设。还讨论了如何避免最常遇到的问题和工件。©2017 by John Wiley & Sons, Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Measuring Protein Interactions by Optical Biosensors.

This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is placed on description of robust approaches that will provide reliable results with few assumptions. How to avoid the most commonly encountered problems and artifacts is also discussed. © 2017 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Protein Science
Current Protocols in Protein Science Biochemistry, Genetics and Molecular Biology-Biochemistry
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期刊介绍: With the mapping of the human genome, more and more researchers are exploring protein structures and functions in living organisms. Current Protocols in Protein Science provides protein scientists, biochemists, molecular biologists, geneticists, and others with the first comprehensive suite of protocols for this growing field.
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