Tdcp Gunasekara, Ndm Radhika, K K Ragunathan, Dpp Gunathilaka, M M Weerasekera, Hgsp Hewageegana, L A D M Arawwawala, Ssn Fernando
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Schneid. and fruit of <i>Garcina zeylanica</i> Roxb.</p><p><strong>Subjects and methods: </strong>Aqueous and ethanolic extracts of dried bark of <i>Pongamia pinnata</i> (Magul karanda), dried stem of <i>Rubia cordifolia Linn</i> (Welmadata), tender leaves of <i>Jasminum officinale Linn</i> (Jasmine) and dried stem of <i>Berberis ceylanica</i> (Dāruharidrā) were prepared according to standard protocols and tested for antimicrobial activity against five clinical isolates and one standard strain each of <i>Candida albicans</i> (ATCC 10231), <i>Candida parapsilosis</i> (ATCC 22019) and six Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) clinical isolates using the well diffusion method. Experiments were done in triplicates using well diffusion method. The plant extracts which gave a zone of inhibition in the well diffusion assay were further tested for Minimum Inhibitory Concentrations (MIC).</p><p><strong>Results: </strong>Aqueous and ethanolic extracts of <i>Berberis ceylanica</i> and ethanolic extract of <i>Rubia cordifolia</i> had antimicrobial activity against <i>Candida albicans</i> and <i>Candida parapsilosis</i>. Aqueous and ethanolic extracts of <i>Garcinia zeylanica</i>, and the ethanolic extracts of <i>Jasminum officinale</i>, <i>Rubia cordifolia</i> and <i>Pongamia pinnata</i> had antimicrobial activity against MRSA.</p><p><strong>Conclusions: </strong><i>Berberis ceylanica</i> and <i>Rubia crodifolia</i> had antimicrobial activity against <i>Candida</i> species while <i>Garcinia zeylanica, Jasminum officinale, Rubia crodifolia</i> and <i>Pongamia pinnata</i> had antimicrobial activity against MRSA.</p>","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"36 4","pages":"187-190"},"PeriodicalIF":0.0000,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5726184/pdf/","citationCount":"10","resultStr":"{\"title\":\"Determination of Antimicrobial Potential of Five Herbs used in Ayurveda Practices against <i>Candida albicans, Candida parapsilosis</i> and Methicillin Resistant <i>Staphylococcus aureus</i>.\",\"authors\":\"Tdcp Gunasekara, Ndm Radhika, K K Ragunathan, Dpp Gunathilaka, M M Weerasekera, Hgsp Hewageegana, L A D M Arawwawala, Ssn Fernando\",\"doi\":\"10.4103/asl.ASL_179_16\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Medicinal plants are an important source of novel antimicrobial agents. Ayurvedic treatment involves the use of a variety of medicinal plants that merit investigation.</p><p><strong>Aims: </strong>To investigate the antimicrobial activity of bark of <i>Pongamia pinnata</i> (L.) Pierre, stem of <i>Rubia cordifolia</i> Linn, leaves of <i>Jasminum officinale</i> Linn, stem of <i>Berberis ceylanica</i> C.K. Schneid. and fruit of <i>Garcina zeylanica</i> Roxb.</p><p><strong>Subjects and methods: </strong>Aqueous and ethanolic extracts of dried bark of <i>Pongamia pinnata</i> (Magul karanda), dried stem of <i>Rubia cordifolia Linn</i> (Welmadata), tender leaves of <i>Jasminum officinale Linn</i> (Jasmine) and dried stem of <i>Berberis ceylanica</i> (Dāruharidrā) were prepared according to standard protocols and tested for antimicrobial activity against five clinical isolates and one standard strain each of <i>Candida albicans</i> (ATCC 10231), <i>Candida parapsilosis</i> (ATCC 22019) and six Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) clinical isolates using the well diffusion method. Experiments were done in triplicates using well diffusion method. The plant extracts which gave a zone of inhibition in the well diffusion assay were further tested for Minimum Inhibitory Concentrations (MIC).</p><p><strong>Results: </strong>Aqueous and ethanolic extracts of <i>Berberis ceylanica</i> and ethanolic extract of <i>Rubia cordifolia</i> had antimicrobial activity against <i>Candida albicans</i> and <i>Candida parapsilosis</i>. Aqueous and ethanolic extracts of <i>Garcinia zeylanica</i>, and the ethanolic extracts of <i>Jasminum officinale</i>, <i>Rubia cordifolia</i> and <i>Pongamia pinnata</i> had antimicrobial activity against MRSA.</p><p><strong>Conclusions: </strong><i>Berberis ceylanica</i> and <i>Rubia crodifolia</i> had antimicrobial activity against <i>Candida</i> species while <i>Garcinia zeylanica, Jasminum officinale, Rubia crodifolia</i> and <i>Pongamia pinnata</i> had antimicrobial activity against MRSA.</p>\",\"PeriodicalId\":7805,\"journal\":{\"name\":\"Ancient Science of Life\",\"volume\":\"36 4\",\"pages\":\"187-190\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5726184/pdf/\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ancient Science of Life\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/asl.ASL_179_16\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ancient Science of Life","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/asl.ASL_179_16","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
摘要
背景:药用植物是新型抗菌药物的重要来源。阿育吠陀治疗包括使用各种值得研究的药用植物。目的:研究桄榔子皮的抑菌活性。皮埃尔,堇青花茎,茉莉花叶,小檗茎。和栀子的果实。研究对象和方法:根据标准方案制备了桄榔子(Magul karanda)干树皮、Rubia cordifolia Linn (Welmadata)干茎、Jasminum officinale Linn (Jasmine)嫩叶和Berberis ceylanica (Dāruharidrā)干茎的水提取物和乙醇提取物,并对白色念珠菌(ATCC 10231)的5个临床分离株和1个标准菌株进行了抗菌活性测试。利用孔扩散法对拟合假丝酵母(ATCC 22019)和6株耐甲氧西林金黄色葡萄球菌(MRSA)临床分离株进行检测。实验采用孔扩散法,分三次进行。对在孔扩散法中具有抑制区的植物提取物进行了最低抑制浓度(MIC)测试。结果:小檗水提液、乙醇提液和风铃草乙醇提液对白色念珠菌和假丝酵母菌均有抑菌活性。栀子花水提物和乙醇提物以及栀子花、风铃花和凤尾花乙醇提物对MRSA均有抑菌活性。结论:大黄小檗和大黄小檗对念珠菌有抑菌活性,栀子、栀子、大黄小檗和小红花对MRSA有抑菌活性。
Determination of Antimicrobial Potential of Five Herbs used in Ayurveda Practices against Candida albicans, Candida parapsilosis and Methicillin Resistant Staphylococcus aureus.
Background: Medicinal plants are an important source of novel antimicrobial agents. Ayurvedic treatment involves the use of a variety of medicinal plants that merit investigation.
Aims: To investigate the antimicrobial activity of bark of Pongamia pinnata (L.) Pierre, stem of Rubia cordifolia Linn, leaves of Jasminum officinale Linn, stem of Berberis ceylanica C.K. Schneid. and fruit of Garcina zeylanica Roxb.
Subjects and methods: Aqueous and ethanolic extracts of dried bark of Pongamia pinnata (Magul karanda), dried stem of Rubia cordifolia Linn (Welmadata), tender leaves of Jasminum officinale Linn (Jasmine) and dried stem of Berberis ceylanica (Dāruharidrā) were prepared according to standard protocols and tested for antimicrobial activity against five clinical isolates and one standard strain each of Candida albicans (ATCC 10231), Candida parapsilosis (ATCC 22019) and six Methicillin Resistant Staphylococcus aureus (MRSA) clinical isolates using the well diffusion method. Experiments were done in triplicates using well diffusion method. The plant extracts which gave a zone of inhibition in the well diffusion assay were further tested for Minimum Inhibitory Concentrations (MIC).
Results: Aqueous and ethanolic extracts of Berberis ceylanica and ethanolic extract of Rubia cordifolia had antimicrobial activity against Candida albicans and Candida parapsilosis. Aqueous and ethanolic extracts of Garcinia zeylanica, and the ethanolic extracts of Jasminum officinale, Rubia cordifolia and Pongamia pinnata had antimicrobial activity against MRSA.
Conclusions: Berberis ceylanica and Rubia crodifolia had antimicrobial activity against Candida species while Garcinia zeylanica, Jasminum officinale, Rubia crodifolia and Pongamia pinnata had antimicrobial activity against MRSA.