一种评估接触过敏原致敏诱导水平的体外方法的建立

Valentina Galbiati, Sue Gibbs, Erwin Roggen, Emanuela Corsini
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引用次数: 16

摘要

最近,研究人员开发并验证了一种体外程序,该程序将表皮等效效力测定与IL-18评估相结合,为皮肤致敏剂的鉴定和分类提供了一种单一的测试。本单元将描述一种简单的体外方法,用于估计预期的致敏诱导水平,通过体外EC50和IL-18 SI2值来预测LLNA EC3和/或人类NOEL,该方法基于使用重组人表皮(RhE)生成的参考接触过敏原标准曲线。©2018 by John Wiley &儿子,Inc。
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Development of an In Vitro Method to Estimate the Sensitization Induction Level of Contact Allergens

Recently, an in vitro procedure, which combines the epidermal equivalent potency assay with assessment of IL-18 to provide a single test for identification and classification of skin sensitizers, was developed and validated. This unit will describe a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL-18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens, based on the use of Reconstituted human Epidermis (RhE).© 2018 by John Wiley & Sons, Inc.

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