MRI评估神经功能

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2018-05-22 DOI:10.1002/cpmo.44
Brian J. Nieman, Matthijs C. van Eede, Shoshana Spring, Jun Dazai, R. Mark Henkelman, Jason P. Lerch
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引用次数: 27

摘要

本文详细介绍了一套使用MRI进行小鼠脑成像的方案。我们主要专注于测量神经解剖学的变化,并提供小鼠准备和图像采集,图像处理和统计的详细说明。提供了实际细节和理论考虑。©2018 by John Wiley &儿子,Inc。
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MRI to Assess Neurological Function

This article describes a detailed set of protocols for mouse brain imaging using MRI. We focus primarily on measuring changes in neuroanatomy, and provide both instructions for mouse preparation and details on image acquisition, image processing, and statistics. Practical details as well as theoretical considerations are provided. © 2018 by John Wiley & Sons, Inc.

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来源期刊
Current protocols in mouse biology
Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology
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期刊介绍: Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.
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