[内吞途径和肝磷脂在细胞内的分布]。

药学学报 Pub Date : 2017-03-01

Huan-huan Peng, Ying Li, Jia-yi Yuan, Jing-xiao Chen, Jing-hua Chen

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引用次数: 0

摘要

本研究研究了肝磷脂聚糖在MCF-7肿瘤细胞和COS7正常细胞中的内吞途径及其在细胞内的分布。内吞抑制和细胞预定位实验表明，MCF-7肿瘤细胞比cos7细胞更有效和选择性地摄取肝磷脂糖。在MCF-7肿瘤细胞和COS7正常细胞中，肝磷脂的细胞摄取都是能量依赖性的。此外，肝素聚糖进入MCF-7肿瘤细胞的主要内吞途径是小窝蛋白介导的内吞作用和巨噬作用。内化肝磷脂主要位于细胞溶酶体中。

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[Endocytosis pathway and intracellular distribution of heparosan].

In this study, the endocytosis pathway of heparosan and its intracellular distribution were investigated in MCF-7 tumor cells and COS7 normal cells. The endocytosis inhibition and cellular probe location experiments showed that MCF-7 tumor cells took heparosan more efficiently and selectively than COS7 cells. The cellular uptake of heparosan was energy-dependent in both MCF-7 tumor cells and COS7 normal cells. Moreover, the major endocytosis pathway of heparosan into MCF-7 tumor cells was caveolin-mediated endocytosis and macropinocytosis. The internalized heparosan was mainly located in lysosomes of the cells.

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来源期刊

药学学报 Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Acta Pharmaceutica Sinica B (APSB) is a bimonthly English peer-reviewed online journal in ScienceDirect, which publishes significant original research articles, communications and high quality reviews of recent advances. APSB encourages submissions from all areas of pharmaceutical sciences, including pharmacology, pharmaceutics, medicinal chemistry, natural products, pharmacognosy, pharmaceutical analysis and pharmacokinetics. APSB is a part of the series Acta Pharmaceutica Sinica, which was founded in 1953. The journal is co-published by Elsevier B.V., in association with the Institute of MateriaMedica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association.