[HPLC-CAD -化学计量学相结合的Toosendan Fructus色谱指纹图谱分析]。

药学学报 Pub Date : 2017-03-01

Chun-ni Zhang, Ying-zi Wang, Xin-guang Sun, Yang Zhao, Wei Zheng, Wen-hua Li, Zhen Long, Bai-ping Ma

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引用次数: 0

摘要

建立了一种利用高效液相色谱-带电气溶胶检测器(CAD)对桃仙丹药材进行指纹图谱分析的新方法。样品在Agilent ZOBAX SB C18色谱柱(4.6 mm × 250 mm, 5 μm)上，以含0.1%甲酸(v/v)的乙腈和水梯度洗脱，流速为1.0 mL·min - 1。柱温为30℃，进样量为5 μL。带电气溶胶探测器(CAD)的氮气入口压力为35psi，雾化器室温度为35℃。此外，色谱指纹图谱结合多元统计分析的方法有效合理，对20批不同产地的样品进行了准确的分类。结果表明，指纹图谱中有28个共同峰，样品可分为3个聚类。该方法具有精密度高、重复性好、稳定性好等特点。该方法可用于赤芍药材的质量控制和价值鉴定。

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[Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods].

A new method was developed for the chromatographic fingerprint analysis of Toosendan Fructus by HPLC coupled with the charged aerosol detector (CAD) in the present study. Samples were well separated on an Agilent ZOBAX SB C18 column (4.6 mm × 250 mm, 5 μm) by gradient elution using acetonitrile and water containing 0.1 % formic acid (v/v) at the flow rate of 1.0 mL·min−1. The column temperature was 30 ℃ and the injection volume was 5 μL. The nitrogen inlet pressure of the charged aerosol detector (CAD) was 35 psi, and the nebulizer chamber temperature was 35 ℃. In addition, the method of the chromatographic fingerprints combined with multivariate statistical analysis was effective and reasonable lead to an accurate classification of 20 batches of samples from different locations. The results showed that 28 common peaks were observed in the fingerprint and the samples were classified into three clusters. The established method was well validated, and showed high precision, good repeatability, and satisfactory stability. It may serve in the quality control and evaluation of Toosendan Fructus.

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来源期刊

药学学报 Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Acta Pharmaceutica Sinica B (APSB) is a bimonthly English peer-reviewed online journal in ScienceDirect, which publishes significant original research articles, communications and high quality reviews of recent advances. APSB encourages submissions from all areas of pharmaceutical sciences, including pharmacology, pharmaceutics, medicinal chemistry, natural products, pharmacognosy, pharmaceutical analysis and pharmacokinetics. APSB is a part of the series Acta Pharmaceutica Sinica, which was founded in 1953. The journal is co-published by Elsevier B.V., in association with the Institute of MateriaMedica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association.