肌肉干细胞免疫染色

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2018-08-14 DOI:10.1002/cpmo.47

Shuaiyu Wang, Bao Zhang, Gregory C. Addicks, Hui Zhang, Keir J. Menzies, Hongbo Zhang

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引用次数: 4

摘要

肌肉干细胞(MuSCs)通过为损伤后和肌肉疾病中的肌肉再生提供祖细胞，对维持肌肉稳态至关重要。MuSC的特性动态变化，反映生理或病理状态。例如，musc在肌肉损伤后被激活，但在杜氏肌营养不良症(DMD)的晚期被耗尽，并在衰老过程中衰老。因此，研究MuSC的增殖、活化、衰老和凋亡等特性，对于将MuSC知识应用于再生医学，如治疗DMD和改善衰老过程中的肌肉功能具有重要意义。在这里，我们描述了一种使用小鼠免疫染色技术在原位表征MuSCs的详细方法。这种方法也可以很容易地用于分析其他骨骼肌特性。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Muscle Stem Cell Immunostaining

Muscle stem cells (MuSCs) are essential for maintaining muscle homeostasis by providing progenitor cells for muscle regeneration after injury and in muscular diseases. MuSC properties dynamically change, reflecting physiology or pathological status. For instance, MuSCs are activated after muscle injury, but become exhausted in late stages of Duchenne Muscular Dystrophy (DMD) disease and senescent during aging. Therefore, characterization of MuSCs, including proliferation, activation, senescence, and apoptosis, etc., is very important in applying MuSC knowledge to regenerative medicine, such as in the treatment of DMD and to improve muscle function in aging. Here, we describe a detailed method for characterizing MuSCs in situ using immunostaining techniques in the mouse. This method can also be easily adapted to analyze other skeletal muscle properties. © 2018 by John Wiley & Sons, Inc.

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.