[Experiment on Jianpi Qingre Huoxue Decoction Drug Serum Regulates SW480 Cell Proliferation,Apoptosis,Cycle and the Expression of β-Catenin by Altering PI3K/Akt Signal Pathway].

Objective: To study Jianpi Qingre Huoxue decoction( JPQRHX) in preventing colon cancer by observing SW480 cells proliferation,apoptosis,cycle and the expression of P-β-catenin, and to research its mechanism.

Methods: SW480 cells were incubated with serum containing blank serum, different concentrations of JPQRHX decocition and PI3 K blocking agent LY294002 for 24 h,respectively. Cell proliferation was detected by MTT assay, cell cycle and apoptosis were detected by flow cytometry. The protein translocation of P-β-catenin was assayed by immunofluorescent staining.

Results: The inhibitory rate and apoptosis rate in JPQRHX decoction group were higher than control group( P < 0. 05),respectively. S phase cells were increased significantly, and G1 phase cells and LY294002 group cells were decreased significantly( P < 0. 05). The P-β-catenin in JPQRHX decoction groups were mainly expressed in membrane, while the P-β-catenin in the control group was characterized by deletion in membrane and increased in nucleus.

Conclusion: JPQRHX decoction has the ability in curing colon cancer, and the mechanism is associated with altering the expression of P-β-catenin in the cells nucleus, blocking SW480 cells cycle at G1 phase, inhibiting SW480 cells proliferation, and inducing SW480 cell apoptosis.