[UPLC-Q-TOF-MS/MS法鉴定刺五加叶片化学成分]。

中药材 Pub Date : 2016-07-01
Hong-chang Cheng, Wen-feng Wei, Jin-hai Huo, Guo-dong Sun, Wei-ming Wang
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引用次数: 0

摘要

目的:对刺五加叶片的化学成分进行分析。方法:采用UPLC-Q-TOF-MS / MS对刺五加叶片中的化学成分进行快速鉴定。采用Peakview数据处理软件对化学成分进行鉴定和推测,检测其保留时间、精确相对分子质量和MS / MS裂解片段。色谱-质谱分析条件如下,色谱柱为Waters BEH c18 (100 mm × 2)。1毫米,1。7 μm)梯度洗脱,流动相为0。1%甲酸水溶液和0。1%甲酸乙腈,流速为0。3 m L / min,采用ESI离子源采用负离子模式和正离子模式采集数据。结果:通过MS / MS、参考文献和Chemispider数据库对30个化合物进行了鉴定和推测。结论:该方法快速、灵敏、全面,可快速鉴别刺五加叶中的化学成分,为完善刺五加叶的质量标准、明确刺五加叶的药效物质基础提供依据。
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[Identification of Chemical Constituents of the Leaves from Acanthopanax senticosus by UPLC-Q-TOF-MS/MS].

Objective: To analyze the chemical compositions of the leaves from Acanthopanax senticosus.

Methods: Rapid identification of chemical constituents in the leaves of Acanthopanax senticosus by UPLC-Q-TOF-MS / MS. The chemical constituents were identified and speculated by using Peakview data processing software, the retention time, exact relative molecular mass, and cleavage fragments of MS / MS were detected. Chromatography-mass spectrometry conditions were as follows, the analysis was performed on Waters BEH C18column( 100 mm × 2. 1 mm,1. 7 μm) in gradient elution with a mobile phase of 0. 1% formic acid aqueous solution and 0. 1%formic acid acetonitrile, the flow rate was at 0. 3 m L / min, the data was collected by the negative and positive ion mode using ESI ion source.

Results: 30 compounds were identified and speculated by the standards and compounds of MS / MS, the references and Chemispider database.

Conclusion: This method is fast, sensitive and comprehensive with the rapid identification of chemical constituents in the leaves of Acanthopanax senticosus, which will provide the evidences for perfecting the quality standard, and clarify the efficacy material base of the leaves of Acanthopanax senticosus.

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来源期刊
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期刊介绍: "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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