Si-you Chen, Rong Zhao, Liang Xu, Quan-jun Feng, Bing Wang, Ting-quo Kang
{"title":"蒙古药用植物黄芩的DNA条形码研究。","authors":"Si-you Chen, Rong Zhao, Liang Xu, Quan-jun Feng, Bing Wang, Ting-quo Kang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To authenticate Scutellaria scordifolia and its closely related species using ITS2 sequence.</p><p><strong>Methods: </strong>Total genomic DNA was isolated from Scutellaria scordifolia and its related species. Nuclear DNA ITS2 sequences were amplified and sequenced,and ITS2 sequences of the other species of plants were obtained in Gen Bank,The Kimura 2-parameter( K2P) distances were calculated. Identification analyses were performed using Neighbor-joining( NJ) and secondary structure of ITS2 sequence methods.</p><p><strong>Results: </strong>The genetic distances of ITS2 between Scutellaria scordifolia and its closely related species were 0. 014 ~ 0. 141,which were obviously higher than those in the intra-species of Scutellaria scordifolia. The NJ tree and secondary structure of ITS2 sequence can distinguish Scutellaria scordifolia and its closely related species.</p><p><strong>Conclusion: </strong>ITS2 sequence can effectively and accurately identify the Mongolian medicinal plant Scutellaria scordifolia and its closely relatives.</p>","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[DNA Barcoding of Mongolian Medicinal Plant Scutellaria scordifolia].\",\"authors\":\"Si-you Chen, Rong Zhao, Liang Xu, Quan-jun Feng, Bing Wang, Ting-quo Kang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To authenticate Scutellaria scordifolia and its closely related species using ITS2 sequence.</p><p><strong>Methods: </strong>Total genomic DNA was isolated from Scutellaria scordifolia and its related species. Nuclear DNA ITS2 sequences were amplified and sequenced,and ITS2 sequences of the other species of plants were obtained in Gen Bank,The Kimura 2-parameter( K2P) distances were calculated. Identification analyses were performed using Neighbor-joining( NJ) and secondary structure of ITS2 sequence methods.</p><p><strong>Results: </strong>The genetic distances of ITS2 between Scutellaria scordifolia and its closely related species were 0. 014 ~ 0. 141,which were obviously higher than those in the intra-species of Scutellaria scordifolia. The NJ tree and secondary structure of ITS2 sequence can distinguish Scutellaria scordifolia and its closely related species.</p><p><strong>Conclusion: </strong>ITS2 sequence can effectively and accurately identify the Mongolian medicinal plant Scutellaria scordifolia and its closely relatives.</p>\",\"PeriodicalId\":15312,\"journal\":{\"name\":\"中药材\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中药材\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[DNA Barcoding of Mongolian Medicinal Plant Scutellaria scordifolia].
Objective: To authenticate Scutellaria scordifolia and its closely related species using ITS2 sequence.
Methods: Total genomic DNA was isolated from Scutellaria scordifolia and its related species. Nuclear DNA ITS2 sequences were amplified and sequenced,and ITS2 sequences of the other species of plants were obtained in Gen Bank,The Kimura 2-parameter( K2P) distances were calculated. Identification analyses were performed using Neighbor-joining( NJ) and secondary structure of ITS2 sequence methods.
Results: The genetic distances of ITS2 between Scutellaria scordifolia and its closely related species were 0. 014 ~ 0. 141,which were obviously higher than those in the intra-species of Scutellaria scordifolia. The NJ tree and secondary structure of ITS2 sequence can distinguish Scutellaria scordifolia and its closely related species.
Conclusion: ITS2 sequence can effectively and accurately identify the Mongolian medicinal plant Scutellaria scordifolia and its closely relatives.
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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