[Analysis of Volatile Components and Special Aroma in Fresh Roots of Codonopsis Radix].

Objective: To analyze volatile components and special aroma in fresh roots of Codonopsis Radix.

Methods: Volatile components in fresh Codonopsis Radix origined from three plant sources were analyzed by HS-GC-MS, and n-hexanal, 2-hexenal and n-hexanol were quantified by HS-GC.

Results: 64 constituents have been identified in Codonopsis Radix,which containing ten alcohol,20 aldehyde,14 terpene, three ketone, four ester, four furan, three acid, one olefin, one alkane, two sulfide and two other compounds. The total percentage of n-hexanal,2-hexenal and n-hexanol was large, which were the main volatile compounds in Codonopsis Radix. The content of n-hexanal in Lu Dangshen, Baitiao Dangshen, Wen Dangshen, Feng Dangshen and Banqiao Dangshen were 54. 89%,59. 01%,55. 07%,42. 42% and 56. 74%,which were the major volatile component contributing to special aroma in Codonopsis Radix. 61 compounds have been identified in Lu Dangshen including 8 specific compounds. An external calibration method was established for simultaneous determination of n-hexanal,2-hexenal and hexanol in Codonopsis Radix. The results showed that all calibration curve three volatile components exhibited good linearity at a range of concentrations( R2≥0. 9995). The recoveries of n-hexanal, 2-hexenal and hexanol were 100. 1%,94. 8% and 112. 6%,and the RSD were 4. 4%,8. 7% and 8. 1%,respectively. The analysis suggested that the content of n-hexanal in Lu Dangshen was 200. 41 μg / g and significantly higher than that in Banqiao Dangshen, Wen Dangshen and Feng Dangshen while similar to Baitiao Dangshen. The content of hexanol in three-year-old Lu Dangshen was significantly higher than that in twoyear-old Lu Dangshen.

Conclusion: The present research indicates that special aroma is an important feature of authentic Dangshen.