{"title":"七叶皂苷钠通过抑制JAK-1/STAT-1信号通路诱导BGC-823和AGS细胞凋亡","authors":"","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism of sodium aescinate on the apoptosis of gastric cancer BGC-823 cells and AGS cells.</p><p><strong>Methods: </strong>CCK-8 assay was used to detect the cell viability of cancer cells; cells morphological changes were observed by inverted microscope; changes in nuclear morphology were observed by fluorescence inverted microscope after DAPI staining; cells apoptosis rate was detected by flow cytometry; Western blotting was used to detected the phosphorylation of JAK-1 and STAT-1; laser confocal microscopy was used to observed the nuclear translocation of STAT-1.</p><p><strong>Results: </strong>Sodium aescinate inhibited cells proliferation in a concentration dependent manner, and changed the cells morphology and nuclear morphology, significantly up-regulated the apoptosis rate of gastric cancer BGC-823 cells and AGS cells, and down-regulated the phosphorylation of JAK-1, STAT-1 protein, inhibited the nuclear translocation of STAT-1.</p><p><strong>Conclusion: </strong>Sodium aescinate inhibit the proliferation and induce apoptosis in the gastric cancer BGC-823 cells and AGS cells, and this process can be implemented by inhibiting the JAK-1/STAT-1 signaling pathway.</p>","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Sodium Aescinate Induced Apoptosis of BGC-823 and AGS Cells by Inhibiting JAK-1/STAT-1 Signaling Pathway].\",\"authors\":\"\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the mechanism of sodium aescinate on the apoptosis of gastric cancer BGC-823 cells and AGS cells.</p><p><strong>Methods: </strong>CCK-8 assay was used to detect the cell viability of cancer cells; cells morphological changes were observed by inverted microscope; changes in nuclear morphology were observed by fluorescence inverted microscope after DAPI staining; cells apoptosis rate was detected by flow cytometry; Western blotting was used to detected the phosphorylation of JAK-1 and STAT-1; laser confocal microscopy was used to observed the nuclear translocation of STAT-1.</p><p><strong>Results: </strong>Sodium aescinate inhibited cells proliferation in a concentration dependent manner, and changed the cells morphology and nuclear morphology, significantly up-regulated the apoptosis rate of gastric cancer BGC-823 cells and AGS cells, and down-regulated the phosphorylation of JAK-1, STAT-1 protein, inhibited the nuclear translocation of STAT-1.</p><p><strong>Conclusion: </strong>Sodium aescinate inhibit the proliferation and induce apoptosis in the gastric cancer BGC-823 cells and AGS cells, and this process can be implemented by inhibiting the JAK-1/STAT-1 signaling pathway.</p>\",\"PeriodicalId\":15312,\"journal\":{\"name\":\"中药材\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中药材\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Sodium Aescinate Induced Apoptosis of BGC-823 and AGS Cells by Inhibiting JAK-1/STAT-1 Signaling Pathway].
Objective: To investigate the mechanism of sodium aescinate on the apoptosis of gastric cancer BGC-823 cells and AGS cells.
Methods: CCK-8 assay was used to detect the cell viability of cancer cells; cells morphological changes were observed by inverted microscope; changes in nuclear morphology were observed by fluorescence inverted microscope after DAPI staining; cells apoptosis rate was detected by flow cytometry; Western blotting was used to detected the phosphorylation of JAK-1 and STAT-1; laser confocal microscopy was used to observed the nuclear translocation of STAT-1.
Results: Sodium aescinate inhibited cells proliferation in a concentration dependent manner, and changed the cells morphology and nuclear morphology, significantly up-regulated the apoptosis rate of gastric cancer BGC-823 cells and AGS cells, and down-regulated the phosphorylation of JAK-1, STAT-1 protein, inhibited the nuclear translocation of STAT-1.
Conclusion: Sodium aescinate inhibit the proliferation and induce apoptosis in the gastric cancer BGC-823 cells and AGS cells, and this process can be implemented by inhibiting the JAK-1/STAT-1 signaling pathway.
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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