Amit Kr Chakraborty, Priyanka Mukherjee, Amarjit Karam, Samir Das, Luit Barkalita, Kekungo Puro, Rajkumari Sanjukta, Sandeep Ghatak, Ingudam Sakuntala, Ram Gopal Laha, Prabodh Borah, S V Ngachan, Indu Sharma, Arnab Sen
{"title":"印度东北部猪BVDV的证据-遗传谱和特征。","authors":"Amit Kr Chakraborty, Priyanka Mukherjee, Amarjit Karam, Samir Das, Luit Barkalita, Kekungo Puro, Rajkumari Sanjukta, Sandeep Ghatak, Ingudam Sakuntala, Ram Gopal Laha, Prabodh Borah, S V Ngachan, Indu Sharma, Arnab Sen","doi":"10.2174/1874357901812010110","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.</p><p><strong>Methods and material: </strong>The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5' UTR.</p><p><strong>Results: </strong>Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5' UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.</p>","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"12 ","pages":"110-120"},"PeriodicalIF":0.0000,"publicationDate":"2018-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142659/pdf/","citationCount":"4","resultStr":"{\"title\":\"Evidence of BVDV in Pigs from North Eastern Part of India- Genetic Profiling and Characterisation.\",\"authors\":\"Amit Kr Chakraborty, Priyanka Mukherjee, Amarjit Karam, Samir Das, Luit Barkalita, Kekungo Puro, Rajkumari Sanjukta, Sandeep Ghatak, Ingudam Sakuntala, Ram Gopal Laha, Prabodh Borah, S V Ngachan, Indu Sharma, Arnab Sen\",\"doi\":\"10.2174/1874357901812010110\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.</p><p><strong>Methods and material: </strong>The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5' UTR.</p><p><strong>Results: </strong>Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5' UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.</p>\",\"PeriodicalId\":23111,\"journal\":{\"name\":\"The Open Virology Journal\",\"volume\":\"12 \",\"pages\":\"110-120\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-08-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142659/pdf/\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Open Virology Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874357901812010110\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2018/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Virology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874357901812010110","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Evidence of BVDV in Pigs from North Eastern Part of India- Genetic Profiling and Characterisation.
Introduction: The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.
Methods and material: The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5' UTR.
Results: Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5' UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.
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