[人类免疫缺陷病毒(HIV)抗原抗体联合试验筛选试验的效果及引起假阳性的因素]。

Takako Inoue, Takaaki Goto, Takayuki Ogiwara, Minoru Oohashi, Tomoyuki Ohike, Satomi Kani, Noboru Shinkai, Yukio Wakimoto, Shigeru Sato, Yusuhito Tanaka
{"title":"[人类免疫缺陷病毒(HIV)抗原抗体联合试验筛选试验的效果及引起假阳性的因素]。","authors":"Takako Inoue,&nbsp;Takaaki Goto,&nbsp;Takayuki Ogiwara,&nbsp;Minoru Oohashi,&nbsp;Tomoyuki Ohike,&nbsp;Satomi Kani,&nbsp;Noboru Shinkai,&nbsp;Yukio Wakimoto,&nbsp;Shigeru Sato,&nbsp;Yusuhito Tanaka","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to evaluate the performance of an HIV antigen-antibody combination assay (fourth-generation) by comparing it with second generation assays that detect anti-HIV.</p><p><strong>Methods: </strong>A total of 105,439 HIV screening tests were performed from January 2004 to March 2015; the second - and fourth generation assays were used for 75,302 and 30,137 samples, respectively. Samples positive on a screening test were confirmed by anti-HIV-1 western blotting (WB) and nucleic acid amplification. By the results of confirmation tests, the efficacies of the second and fourth generation assays were estimated. The clinical backgrounds with false-positive samples were examined.</p><p><strong>Results: </strong>Of 75,302 samples, 136(0.18%) were positive by the second-generation assay; 14 were confirmed positives, and 122 were false positives. Of 30,137 samples, 18(0.06%) were positive by the fourth-generation assay; 6 were confirmed positives, and 12 were false positives. The reliability of the positives by fourth-generation assay was significantly improved (p=0.006) Samples form individuals with malignant neoplasms were frequently false positive by both the second and fourth-generation assays. Of 67 samples performed by WB, 10 samples, including 6 from patients with a malignancy, showed indeterminate results. All indeterminate samples were found to have antibodies responding to HIV core protein.</p><p><strong>Conclusion: </strong>The fourth-generation assay had satisfactory reliability of the positives for HIV screening. Antibodies responding to HIV core protein may result in false positive HIV screening tests. [Original]</p>","PeriodicalId":21457,"journal":{"name":"Rinsho byori. The Japanese journal of clinical pathology","volume":"64 12","pages":"1335-40"},"PeriodicalIF":0.0000,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Efficacy of Human Immunodeficiency Virus (HIV) Antigen-Antibody Combination Assay as a Screening Test and Factors Causing False-Positivity].\",\"authors\":\"Takako Inoue,&nbsp;Takaaki Goto,&nbsp;Takayuki Ogiwara,&nbsp;Minoru Oohashi,&nbsp;Tomoyuki Ohike,&nbsp;Satomi Kani,&nbsp;Noboru Shinkai,&nbsp;Yukio Wakimoto,&nbsp;Shigeru Sato,&nbsp;Yusuhito Tanaka\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>We aimed to evaluate the performance of an HIV antigen-antibody combination assay (fourth-generation) by comparing it with second generation assays that detect anti-HIV.</p><p><strong>Methods: </strong>A total of 105,439 HIV screening tests were performed from January 2004 to March 2015; the second - and fourth generation assays were used for 75,302 and 30,137 samples, respectively. Samples positive on a screening test were confirmed by anti-HIV-1 western blotting (WB) and nucleic acid amplification. By the results of confirmation tests, the efficacies of the second and fourth generation assays were estimated. The clinical backgrounds with false-positive samples were examined.</p><p><strong>Results: </strong>Of 75,302 samples, 136(0.18%) were positive by the second-generation assay; 14 were confirmed positives, and 122 were false positives. Of 30,137 samples, 18(0.06%) were positive by the fourth-generation assay; 6 were confirmed positives, and 12 were false positives. The reliability of the positives by fourth-generation assay was significantly improved (p=0.006) Samples form individuals with malignant neoplasms were frequently false positive by both the second and fourth-generation assays. Of 67 samples performed by WB, 10 samples, including 6 from patients with a malignancy, showed indeterminate results. All indeterminate samples were found to have antibodies responding to HIV core protein.</p><p><strong>Conclusion: </strong>The fourth-generation assay had satisfactory reliability of the positives for HIV screening. Antibodies responding to HIV core protein may result in false positive HIV screening tests. [Original]</p>\",\"PeriodicalId\":21457,\"journal\":{\"name\":\"Rinsho byori. The Japanese journal of clinical pathology\",\"volume\":\"64 12\",\"pages\":\"1335-40\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rinsho byori. The Japanese journal of clinical pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rinsho byori. The Japanese journal of clinical pathology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的:我们旨在通过将第四代HIV抗原-抗体联合检测方法与第二代抗HIV检测方法进行比较来评估其性能。方法:2004年1月至2015年3月共进行105439例HIV筛查;第二代和第四代检测分别用于75,302和30,137个样本。筛选试验阳性的样品经抗hiv -1免疫印迹(WB)和核酸扩增证实。通过确认试验的结果,估计了第二代和第四代测定法的有效性。检查假阳性标本的临床背景。结果:75,302份样本中,136份(0.18%)经第二代检测呈阳性;确诊阳性14例,假阳性122例。在30137份样本中,18份(0.06%)在第四代检测中呈阳性;确诊阳性6例,假阳性12例。第四代检测结果的可靠性显著提高(p=0.006)。恶性肿瘤患者的样本在第二代和第四代检测中都经常出现假阳性。在WB检查的67个样本中,有10个样本,包括6个来自恶性肿瘤患者的样本,结果不确定。所有不确定的样本都被发现有针对HIV核心蛋白的抗体。结论:第四代检测方法对HIV阳性筛查具有满意的可靠性。对HIV核心蛋白有反应的抗体可能导致HIV筛查试验假阳性。(原始)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
[Efficacy of Human Immunodeficiency Virus (HIV) Antigen-Antibody Combination Assay as a Screening Test and Factors Causing False-Positivity].

Purpose: We aimed to evaluate the performance of an HIV antigen-antibody combination assay (fourth-generation) by comparing it with second generation assays that detect anti-HIV.

Methods: A total of 105,439 HIV screening tests were performed from January 2004 to March 2015; the second - and fourth generation assays were used for 75,302 and 30,137 samples, respectively. Samples positive on a screening test were confirmed by anti-HIV-1 western blotting (WB) and nucleic acid amplification. By the results of confirmation tests, the efficacies of the second and fourth generation assays were estimated. The clinical backgrounds with false-positive samples were examined.

Results: Of 75,302 samples, 136(0.18%) were positive by the second-generation assay; 14 were confirmed positives, and 122 were false positives. Of 30,137 samples, 18(0.06%) were positive by the fourth-generation assay; 6 were confirmed positives, and 12 were false positives. The reliability of the positives by fourth-generation assay was significantly improved (p=0.006) Samples form individuals with malignant neoplasms were frequently false positive by both the second and fourth-generation assays. Of 67 samples performed by WB, 10 samples, including 6 from patients with a malignancy, showed indeterminate results. All indeterminate samples were found to have antibodies responding to HIV core protein.

Conclusion: The fourth-generation assay had satisfactory reliability of the positives for HIV screening. Antibodies responding to HIV core protein may result in false positive HIV screening tests. [Original]

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
[Candida]. [Bleeding time]. [Microparticle]. [Aldolase]. [Telomerase].
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1