利用脑内微透析阐明环境诱导的神经毒性机制

Stephen M. Lasley
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引用次数: 1

摘要

微透析技术允许评估神经递质活性和监测组织细胞外液中的其他细胞实体。该方法广泛用于定量生物胺和氨基酸递质、多肽、给药和其他响应于各种实验处理的分子。本文概述了脑内微透析方法在神经毒理学领域的应用,以阐明环境因子的作用。该技术以各种创造性的方式用于解决涉及无数毒物的特定实验目标。在适当考虑方法参数的情况下,研究者也能够在他们的研究中解决机制问题。这些研究包括在不同的环境金属暴露方案后对细胞外液中的神经递质进行采样,以及评估血脑屏障的渗透性,检测活性氧,以及描述环境因子的毒性动力学。本研究的目的不是回顾调查结果本身,而是强调使用该方法的各种方法以及已解决的实验问题。©2019 by John Wiley &儿子,Inc。
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The Use of Intracerebral Microdialysis to Elucidate Environmentally Induced Neurotoxic Mechanisms

The technique of microdialysis permits the assessment of neurotransmitter activity and the monitoring of other cellular entities in tissue extracellular fluid. The method is widely used for quantifying biogenic amine and amino acid transmitters, peptides, administered drugs, and other molecules in response to various experimental treatments. This article provides an overview of the manner in which the methodology of intracerebral microdialysis is utilized in the field of neurotoxicology to elucidate the actions of environmental agents. The technique is employed in a variety of creative ways to address specific experimental goals involving myriad toxicants. With appropriate consideration of method parameters, investigators have also been able to address mechanistic issues in their studies. These investigations consist of sampling of neurotransmitters in extracellular fluid after various protocols of environmental metal exposure as well as assessments of blood–brain barrier permeability, the detection of reactive oxygen species, and description of the toxicodynamics of environmental agents. The purpose of this examination is not to review the investigational findings, per se, but to highlight the various approaches utilized with this methodology and the experimental questions that have been addressed. © 2019 by John Wiley & Sons, Inc.

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