二甲胺四环素增加体外皮质神经元细胞在激光诱导轴切术后的存活率。

IF 3.2 Q2 Pharmacology, Toxicology and Pharmaceutics Current clinical pharmacology Pub Date : 2020-01-01 DOI:10.2174/1574884714666190226093119
Burak Yulug, Mehmet Ozansoy, Merve Alokten, Muzaffer B C Ozansoy, Seyda Cankaya, Lutfu Hanoglu, Ulkan Kilic, Ertugrul Kilic
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引用次数: 1

摘要

背景:针对多种再生机制的抗生素治疗具有潜在的神经保护作用,但实验策略的多样性和非标准化治疗试验的分析是具有挑战性的。在这方面,当涉及到人类患者时,这些候选分子还没有成功的临床应用案例。方法:培养24 h后,在激光轴切开术前15分钟,分别向原代皮质神经元中添加3种不同浓度(1 μM、10 μM和100 μM)的米诺环素(Sigma-Aldrich, M9511, Germany),观察米诺环素在不同剂量下的保护作用。结果:本研究表明,米诺环素在1 μ m和100μM剂量下具有显著的神经保护作用。除了在更标准化和先进的体外创伤模型中证实二甲胺四环素的神经保护作用外,我们的发现可能对未来集中在动物和人类研究之间的翻译阻滞的研究具有重要意义。结论:这种复杂的方法也可能有助于克服人为变异对关键实验损伤模型的影响。据我们所知,这是第一个研究表明二甲胺四环素增加体外神经细胞存活后,激光轴切开术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy.

Background: Antibiotic therapies targeting multiple regenerative mechanisms have the potential for neuroprotective effects, but the diversity of experimental strategies and analyses of non-standardised therapeutic trials are challenging. In this respect, there are no cases of successful clinical application of such candidate molecules when it comes to human patients.

Methods: After 24 hours of culturing, three different minocycline (Sigma-Aldrich, M9511, Germany) concentrations (1 μM, 10 μM and 100 μM) were added to the primary cortical neurons 15 minutes before laser axotomy procedure in order to observe protective effect of minocycline in these dosages.

Results: Here, we have shown that minocycline exerted a significant neuroprotective effect at 1 and 100μM doses. Beyond confirming the neuroprotective effect of minocycline in a more standardised and advanced in-vitro trauma model, our findings could have important implications for future studies that concentrate on the translational block between animal and human studies.

Conclusion: Such sophisticated approaches might also help to conquer the influence of humanmade variabilities in critical experimental injury models. To the best of our knowledge, this is the first study showing that minocycline increases in-vitro neuronal cell survival after laser-axotomy.

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Current clinical pharmacology
Current clinical pharmacology PHARMACOLOGY & PHARMACY-
CiteScore
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期刊介绍: Current Clinical Pharmacology publishes frontier reviews on all the latest advances in clinical pharmacology. The journal"s aim is to publish the highest quality review articles in the field. Topics covered include: pharmacokinetics; therapeutic trials; adverse drug reactions; drug interactions; drug metabolism; pharmacoepidemiology; and drug development. The journal is essential reading for all researchers in clinical pharmacology.
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