大豆过氧化物酶催化酸性偶氮染料脱色。

IF 2.4 Q1 Medicine Journal of Health and Pollution Pub Date : 2020-02-28 eCollection Date: 2020-03-01 DOI:10.5696/2156-9614-10.25.200307
Bahaa Malik Altahir, Teeba Jaffar Al-Robaiey, Zainab Mohammad Abbaas, Neda Mashhadi, Laura G Cordova Villegas, Keith E Taylor, Nihar Biswas
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摘要

背景:一些工业生产过程会产生和排放染料作为水污染物,其中许多是有毒有害物质。因此需要更温和、更环保的染料处理方法:本研究的目的是研究和优化粗制大豆过氧化物酶(SBP)对偶氮染料的脱色效果,研究对象是两种在工业上广泛使用但在结构复杂性上差别很大的染料--酸性黑 2 和酸性橙 7,并研究特定参数对去除过程的影响:方法:使用间歇反应器去除 95% 的染料颜色,并产生大量沉淀物:结果:酸性黑 2 的最佳酶脱色 pH 值为酸性区域(pH 值为 4.4),酸性橙 7 的最佳酶脱色 pH 值为中性条件(pH 值为 6.9)。在 0.5 毫摩尔浓度下,两种染料的最低酶活性均为 1.2 U/mL。要达到约 95% 的去除率,酸性橙 7 和酸性黑 2 的过氧化氢/底物摩尔比分别为 3 和 2.5。对在各自最佳条件下收集的进度曲线数据进行一阶拟合,酸性橙 7 和酸性黑 2 的半衰期分别为 23.9 分钟和 28.9 分钟:成功证明了 SBP 催化处理工业染料酸性黑 2 和/或酸性橙 7 或类似染料的可行性,因为它们可能出现在工业废水中:作者声明不涉及任何经济利益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Soybean Peroxidase Catalyzed Decoloration of Acid Azo Dyes.

Background: Some industrial manufacturing processes generate and release dyes as water pollutants, many of which are toxic and hazardous materials. There is a need for milder, greener methods for dye treatment.

Objectives: The objective of the present study was to investigate and optimize azo dye decoloration by a crude soybean peroxidase (SBP), based on two dyes that have widespread industrial use, but that differ greatly in structural complexity, Acid Black 2 and Acid Orange 7, and to investigate the effects of specific parameters on the removal process.

Methods: Batch reactors were used to remove 95% of the dyes' color and to produce substantial precipitates.

Results: The optimum pH for enzymatic decoloration of Acid Black 2 was in the acidic region, pH 4.4, and that of Acid Orange 7 occurred under neutral conditions, pH 6.9. The minimum enzyme activity needed for sufficient removal was 1.2 U/mL for both dyes at 0.5 mM. The minimum molar hydrogen peroxide/substrate ratio was 3 for Acid Orange 7 and 2.5 for Acid Black 2 to achieve approximately 95% removal. First-order fitting of progress curve data collected under the respective optimum conditions gave half-lives of 23.9 and 28.9 minutes for Acid Orange 7 and Acid Black 2, respectively.

Conclusions: The feasibility of SBP-catalyzed treatment of industrial dyes Acid Black 2 and/or Acid Orange 7, or dyes that resemble them, as they might occur in industrial effluents, was successfully demonstrated.

Competing interests: The authors declare no competing financial interests.

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来源期刊
Journal of Health and Pollution
Journal of Health and Pollution Medicine-Public Health, Environmental and Occupational Health
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审稿时长
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期刊介绍: The Journal of Health and Pollution (JH&P) was initiated with funding from the European Union and World Bank and continues to be a Platinum Open Access Journal. There are no publication or viewing charges. That is, there are no charges to readers or authors. Upon peer-review and acceptance, all articles are made available online. The high-ranking editorial board is comprised of active members who participate in JH&P submissions and editorial policies. The Journal of Health and Pollution welcomes manuscripts based on original research as well as findings from re-interpretation and examination of existing data. JH&P focuses on point source pollution, related health impacts, environmental control and remediation technology. JH&P also has an interest in ambient and indoor pollution. Pollutants of particular interest include heavy metals, pesticides, radionuclides, dioxins, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs), air particulates (PM10 and PM2.5), and other severe and persistent toxins. JH&P emphasizes work relating directly to low and middle-income countries, however relevant work relating to high-income countries will be considered on a case-by-case basis.
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