Bahaa Malik Altahir, Teeba Jaffar Al-Robaiey, Zainab Mohammad Abbaas, Neda Mashhadi, Laura G Cordova Villegas, Keith E Taylor, Nihar Biswas
{"title":"大豆过氧化物酶催化酸性偶氮染料脱色。","authors":"Bahaa Malik Altahir, Teeba Jaffar Al-Robaiey, Zainab Mohammad Abbaas, Neda Mashhadi, Laura G Cordova Villegas, Keith E Taylor, Nihar Biswas","doi":"10.5696/2156-9614-10.25.200307","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Some industrial manufacturing processes generate and release dyes as water pollutants, many of which are toxic and hazardous materials. There is a need for milder, greener methods for dye treatment.</p><p><strong>Objectives: </strong>The objective of the present study was to investigate and optimize azo dye decoloration by a crude soybean peroxidase (SBP), based on two dyes that have widespread industrial use, but that differ greatly in structural complexity, Acid Black 2 and Acid Orange 7, and to investigate the effects of specific parameters on the removal process.</p><p><strong>Methods: </strong>Batch reactors were used to remove 95% of the dyes' color and to produce substantial precipitates.</p><p><strong>Results: </strong>The optimum pH for enzymatic decoloration of Acid Black 2 was in the acidic region, pH 4.4, and that of Acid Orange 7 occurred under neutral conditions, pH 6.9. The minimum enzyme activity needed for sufficient removal was 1.2 U/mL for both dyes at 0.5 mM. The minimum molar hydrogen peroxide/substrate ratio was 3 for Acid Orange 7 and 2.5 for Acid Black 2 to achieve approximately 95% removal. First-order fitting of progress curve data collected under the respective optimum conditions gave half-lives of 23.9 and 28.9 minutes for Acid Orange 7 and Acid Black 2, respectively.</p><p><strong>Conclusions: </strong>The feasibility of SBP-catalyzed treatment of industrial dyes Acid Black 2 and/or Acid Orange 7, or dyes that resemble them, as they might occur in industrial effluents, was successfully demonstrated.</p><p><strong>Competing interests: </strong>The authors declare no competing financial interests.</p>","PeriodicalId":52138,"journal":{"name":"Journal of Health and Pollution","volume":"10 25","pages":"200307"},"PeriodicalIF":2.4000,"publicationDate":"2020-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058142/pdf/","citationCount":"0","resultStr":"{\"title\":\"Soybean Peroxidase Catalyzed Decoloration of Acid Azo Dyes.\",\"authors\":\"Bahaa Malik Altahir, Teeba Jaffar Al-Robaiey, Zainab Mohammad Abbaas, Neda Mashhadi, Laura G Cordova Villegas, Keith E Taylor, Nihar Biswas\",\"doi\":\"10.5696/2156-9614-10.25.200307\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Some industrial manufacturing processes generate and release dyes as water pollutants, many of which are toxic and hazardous materials. There is a need for milder, greener methods for dye treatment.</p><p><strong>Objectives: </strong>The objective of the present study was to investigate and optimize azo dye decoloration by a crude soybean peroxidase (SBP), based on two dyes that have widespread industrial use, but that differ greatly in structural complexity, Acid Black 2 and Acid Orange 7, and to investigate the effects of specific parameters on the removal process.</p><p><strong>Methods: </strong>Batch reactors were used to remove 95% of the dyes' color and to produce substantial precipitates.</p><p><strong>Results: </strong>The optimum pH for enzymatic decoloration of Acid Black 2 was in the acidic region, pH 4.4, and that of Acid Orange 7 occurred under neutral conditions, pH 6.9. The minimum enzyme activity needed for sufficient removal was 1.2 U/mL for both dyes at 0.5 mM. The minimum molar hydrogen peroxide/substrate ratio was 3 for Acid Orange 7 and 2.5 for Acid Black 2 to achieve approximately 95% removal. First-order fitting of progress curve data collected under the respective optimum conditions gave half-lives of 23.9 and 28.9 minutes for Acid Orange 7 and Acid Black 2, respectively.</p><p><strong>Conclusions: </strong>The feasibility of SBP-catalyzed treatment of industrial dyes Acid Black 2 and/or Acid Orange 7, or dyes that resemble them, as they might occur in industrial effluents, was successfully demonstrated.</p><p><strong>Competing interests: </strong>The authors declare no competing financial interests.</p>\",\"PeriodicalId\":52138,\"journal\":{\"name\":\"Journal of Health and Pollution\",\"volume\":\"10 25\",\"pages\":\"200307\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2020-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058142/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Health and Pollution\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5696/2156-9614-10.25.200307\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Health and Pollution","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5696/2156-9614-10.25.200307","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/3/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Soybean Peroxidase Catalyzed Decoloration of Acid Azo Dyes.
Background: Some industrial manufacturing processes generate and release dyes as water pollutants, many of which are toxic and hazardous materials. There is a need for milder, greener methods for dye treatment.
Objectives: The objective of the present study was to investigate and optimize azo dye decoloration by a crude soybean peroxidase (SBP), based on two dyes that have widespread industrial use, but that differ greatly in structural complexity, Acid Black 2 and Acid Orange 7, and to investigate the effects of specific parameters on the removal process.
Methods: Batch reactors were used to remove 95% of the dyes' color and to produce substantial precipitates.
Results: The optimum pH for enzymatic decoloration of Acid Black 2 was in the acidic region, pH 4.4, and that of Acid Orange 7 occurred under neutral conditions, pH 6.9. The minimum enzyme activity needed for sufficient removal was 1.2 U/mL for both dyes at 0.5 mM. The minimum molar hydrogen peroxide/substrate ratio was 3 for Acid Orange 7 and 2.5 for Acid Black 2 to achieve approximately 95% removal. First-order fitting of progress curve data collected under the respective optimum conditions gave half-lives of 23.9 and 28.9 minutes for Acid Orange 7 and Acid Black 2, respectively.
Conclusions: The feasibility of SBP-catalyzed treatment of industrial dyes Acid Black 2 and/or Acid Orange 7, or dyes that resemble them, as they might occur in industrial effluents, was successfully demonstrated.
Competing interests: The authors declare no competing financial interests.
期刊介绍:
The Journal of Health and Pollution (JH&P) was initiated with funding from the European Union and World Bank and continues to be a Platinum Open Access Journal. There are no publication or viewing charges. That is, there are no charges to readers or authors. Upon peer-review and acceptance, all articles are made available online. The high-ranking editorial board is comprised of active members who participate in JH&P submissions and editorial policies. The Journal of Health and Pollution welcomes manuscripts based on original research as well as findings from re-interpretation and examination of existing data. JH&P focuses on point source pollution, related health impacts, environmental control and remediation technology. JH&P also has an interest in ambient and indoor pollution. Pollutants of particular interest include heavy metals, pesticides, radionuclides, dioxins, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs), air particulates (PM10 and PM2.5), and other severe and persistent toxins. JH&P emphasizes work relating directly to low and middle-income countries, however relevant work relating to high-income countries will be considered on a case-by-case basis.