利用综合生物信息学分析鉴定 IgA 肾病的关键基因、通路和潜在治疗药物。

IF 2.1 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Journal of the Renin-Angiotensin-Aldosterone System Pub Date : 2020-04-01 DOI:10.1177/1470320320919635
Xiaoxue Chen, Mindan Sun
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引用次数: 0

摘要

目的:本研究旨在根据芯片数据确定免疫球蛋白-A-肾病相关基因,并研究治疗免疫球蛋白-A-肾病的潜在新基因靶点:免疫球蛋白-A肾病芯片数据来自基因表达总库数据库,其中包括10个免疫球蛋白-A肾病样本和22个正常样本。我们使用 R 软件的 limma 软件包筛选免疫球蛋白-A 肾病和正常肾小球区组织中的差异表达基因。我们对差异表达基因进行了功能富集(包括细胞成分、分子功能、生物过程)和信号通路分析。在线分析数据库(STRING)用于构建差异表达基因的蛋白-蛋白相互作用网络,Cytoscape软件用于识别信号通路的枢纽基因。此外,我们还利用 Connectivity Map 数据库预测了治疗免疫球蛋白-A 肾病的可能药物:结果:共筛选出348个差异表达基因,包括107个上调基因和241个下调基因。功能分析显示,上调差异表达基因主要集中在白细胞迁移方面,而下调差异表达基因则显著富集在α-氨基酸代谢过程中。共获得 6 个中枢基因:JUN、C3AR1、FN1、AGT、FOS 和 SUCNR1。小分子药物thapsigargin、ciclopirox和ikarugamycin被预测为免疫球蛋白-A肾病的治疗靶点:结论:差异表达基因和枢纽基因有助于了解免疫球蛋白-A肾病的分子机制,并为免疫球蛋白-A肾病的诊断和治疗提供潜在的治疗靶点和药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Identification of key genes, pathways and potential therapeutic agents for IgA nephropathy using an integrated bioinformatics analysis.

Purpose: This study aims to identify immunoglobulin-A-nephropathy-related genes based on microarray data and to investigate novel potential gene targets for immunoglobulin-A-nephropathy treatment.

Methods: Immunoglobulin-A-nephropathy chip data was obtained from the Gene Expression Omnibus database, which included 10 immunoglobulin-A-nephropathy and 22 normal samples. We used the limma package of R software to screen differentially expressed genes in immunoglobulin-A-nephropathy and normal glomerular compartment tissues. Functional enrichment (including cellular components, molecular functions, biological processes) and signal pathways were performed for the differentially expressed genes. The online analysis database (STRING) was used to construct the protein-protein interaction networks of differentially expressed genes, and Cytoscape software was used to identify the hub genes of the signal pathway. In addition, we used the Connectivity Map database to predict possible drugs for the treatment of immunoglobulin-A-nephropathy.

Results: A total of 348 differentially expressed genes were screened including 107 up-regulated and 241 down-regulated genes. Functional analysis showed that up-regulated differentially expressed genes were mainly concentrated on leukocyte migration, and the down-regulated differentially expressed genes were significantly enriched in alpha-amino acid metabolic process. A total of six hub genes were obtained: JUN, C3AR1, FN1, AGT, FOS, and SUCNR1. The small-molecule drugs thapsigargin, ciclopirox and ikarugamycin were predicted therapeutic targets against immunoglobulin-A-nephropathy.

Conclusion: Differentially expressed genes and hub genes can contribute to understanding the molecular mechanism of immunoglobulin-A-nephropathy and providing potential therapeutic targets and drugs for the diagnosis and treatment of immunoglobulin-A-nephropathy.

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来源期刊
CiteScore
6.20
自引率
0.00%
发文量
16
审稿时长
6-12 weeks
期刊介绍: JRAAS is a peer-reviewed, open access journal, serving as a resource for biomedical professionals, primarily with an active interest in the renin-angiotensin-aldosterone system in humans and other mammals. It publishes original research and reviews on the normal and abnormal function of this system and its pharmacology and therapeutics, mostly in a cardiovascular context but including research in all areas where this system is present, including the brain, lungs and gastro-intestinal tract.
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