Michael R Hussey, Amber Burt, Maya A Deyssenroth, Brian P Jackson, Ke Hao, Shouneng Peng, Jia Chen, Carmen J Marsit, Todd M Everson
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Of the 1191 lncRNAs sequenced, 46 demonstrated associations (<i>q</i> < 0.05) with BW in models controlling for infant sex, maternal age, BMI, maternal education, and smoking during pregnancy. Furthermore, four of these transcripts were associated with placental cadmium concentrations, with <i>MIR22HG</i> and <i>ERVH48-1</i> demonstrating increases in expression associated with increasing cadmium exposure and elevated odds of small for gestational age birth, while <i>AC114763.2</i> and <i>LINC02595</i> demonstrated reduced expression associated with cadmium, but elevated odds of large for gestational age birth with increasing expression. We identified relationships between lncRNA expression with both placental cadmium concentrations and BW. This study provides evidence that disrupted placental expression of lncRNAs may be a part of cadmium's mechanisms of reproductive toxicity.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2020-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a1/86/dvaa003.PMC7211362.pdf","citationCount":"0","resultStr":"{\"title\":\"Placental lncRNA expression associated with placental cadmium concentrations and birth weight.\",\"authors\":\"Michael R Hussey, Amber Burt, Maya A Deyssenroth, Brian P Jackson, Ke Hao, Shouneng Peng, Jia Chen, Carmen J Marsit, Todd M Everson\",\"doi\":\"10.1093/eep/dvaa003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Heavy metal exposures, such as cadmium, can have negative effects on infant birth weight (BW)-among other developmental outcomes-with placental dysfunction potentially playing a role in these effects. 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Furthermore, four of these transcripts were associated with placental cadmium concentrations, with <i>MIR22HG</i> and <i>ERVH48-1</i> demonstrating increases in expression associated with increasing cadmium exposure and elevated odds of small for gestational age birth, while <i>AC114763.2</i> and <i>LINC02595</i> demonstrated reduced expression associated with cadmium, but elevated odds of large for gestational age birth with increasing expression. We identified relationships between lncRNA expression with both placental cadmium concentrations and BW. 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引用次数: 0
摘要
重金属(如镉)暴露会对婴儿出生体重(BW)及其他发育结果产生负面影响,而胎盘功能障碍可能在这些影响中起到一定作用。在这项研究中,我们研究了胎盘中长非编码 RNA(lncRNA)的不同表达可能与胎盘中的镉水平有何关联,以及这些 lncRNA 的表达差异是否与胎儿的生长有关。在罗德岛儿童健康研究(Rhode Island Child Health Study)中,我们利用Illumina HiSeq全转录组RNA测序数据(n = 199)研究了lncRNA表达与婴儿体重测量以及胎盘镉浓度之间的关系,并控制了适当的协变量。在测序的1191个lncRNA中,46个表现出相关性(q MIR22HG和ERVH48-1表现出与镉暴露增加相关的表达增加以及小胎龄出生几率升高,而AC114763.2和LINC02595表现出与镉相关的表达降低,但随着表达增加,大胎龄出生几率升高。我们确定了 lncRNA 表达与胎盘镉浓度和体重之间的关系。这项研究提供的证据表明,lncRNA的胎盘表达紊乱可能是镉的生殖毒性机制的一部分。
Placental lncRNA expression associated with placental cadmium concentrations and birth weight.
Heavy metal exposures, such as cadmium, can have negative effects on infant birth weight (BW)-among other developmental outcomes-with placental dysfunction potentially playing a role in these effects. In this study, we examined how differential placental expression of long non-coding RNAs (lncRNAs) may be associated with cadmium levels in placenta and whether differences in the expression of those lncRNAs were associated with fetal growth. In the Rhode Island Child Health Study, we used data from Illumina HiSeq whole transcriptome RNA sequencing (n = 199) to examine association between lncRNA expression and measures of infant BW as well as placental cadmium concentrations controlled for appropriate covariates. Of the 1191 lncRNAs sequenced, 46 demonstrated associations (q < 0.05) with BW in models controlling for infant sex, maternal age, BMI, maternal education, and smoking during pregnancy. Furthermore, four of these transcripts were associated with placental cadmium concentrations, with MIR22HG and ERVH48-1 demonstrating increases in expression associated with increasing cadmium exposure and elevated odds of small for gestational age birth, while AC114763.2 and LINC02595 demonstrated reduced expression associated with cadmium, but elevated odds of large for gestational age birth with increasing expression. We identified relationships between lncRNA expression with both placental cadmium concentrations and BW. This study provides evidence that disrupted placental expression of lncRNAs may be a part of cadmium's mechanisms of reproductive toxicity.