兰氏贾第鞭毛虫:实验室维护、生命周期诱导和小鼠模型感染

Current Protocols in Microbiology Pub Date : 2020-06-09 DOI:10.1002/cpmc.102

Marc Y. Fink, Danielle Shapiro, Steven M. Singer

{"title":"兰氏贾第鞭毛虫:实验室维护、生命周期诱导和小鼠模型感染","authors":"Marc Y. Fink, Danielle Shapiro, Steven M. Singer","doi":"10.1002/cpmc.102","DOIUrl":null,"url":null,"abstract":"Giardia lamblia is a protozoan parasite that is found ubiquitously throughout the world and is a major contributor to diarrheal disease. Giardia exhibits a biphasic lifestyle existing as either a dormant cyst or a vegetative trophozoite. Infections are typically initiated through the consumption of cyst-contaminated water or food. Giardia was first axenized in the 1970s and can be readily maintained in a laboratory setting. Additionally, Giardia is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain Giardia and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. © 2020 Wiley Periodicals LLC.Basic Protocol 1: In vitro maintenance and growth of Giardia trophozoitesBasic Protocol 2: In vitro encystation of Giardia cystsBasic Protocol 3: In vivo infections using Giardia trophozoites","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.102","citationCount":"10","resultStr":"{\"title\":\"Giardia lamblia: Laboratory Maintenance, Lifecycle Induction, and Infection of Murine Models\",\"authors\":\"Marc Y. Fink, Danielle Shapiro, Steven M. Singer\",\"doi\":\"10.1002/cpmc.102\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Giardia lamblia is a protozoan parasite that is found ubiquitously throughout the world and is a major contributor to diarrheal disease. Giardia exhibits a biphasic lifestyle existing as either a dormant cyst or a vegetative trophozoite. Infections are typically initiated through the consumption of cyst-contaminated water or food. Giardia was first axenized in the 1970s and can be readily maintained in a laboratory setting. Additionally, Giardia is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain Giardia and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. © 2020 Wiley Periodicals LLC.Basic Protocol 1: In vitro maintenance and growth of Giardia trophozoitesBasic Protocol 2: In vitro encystation of Giardia cystsBasic Protocol 3: In vivo infections using Giardia trophozoites\",\"PeriodicalId\":39967,\"journal\":{\"name\":\"Current Protocols in Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-06-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpmc.102\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.102\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.102","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 10

摘要

贾第鞭毛虫是一种原生动物寄生虫，在世界各地无处不在，是腹泻病的主要原因。贾第鞭毛虫表现出两相的生活方式，要么是休眠的囊肿，要么是营养滋养体。感染通常是通过饮用被囊肿污染的水或食物引起的。贾第鞭毛虫于20世纪70年代首次被消灭，可以在实验室环境中很容易地维持。此外，贾第鞭毛虫是少数可以通过实验室方法诱导完成其完整生命周期的原生动物之一。在本文中，我们概述了维持贾第鞭毛虫并诱导其生命周期传代的方案。我们还提供了在动物感染模型中感染和定量寄生虫的方案。©2020 Wiley期刊有限责任公司基本方案1:贾第鞭毛虫滋养体的体外维持和生长基本方案2:贾第鞭毛虫囊体的体外囊化基本方案3:贾第鞭毛虫滋养体的体内感染

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Giardia lamblia: Laboratory Maintenance, Lifecycle Induction, and Infection of Murine Models

Giardia lamblia is a protozoan parasite that is found ubiquitously throughout the world and is a major contributor to diarrheal disease. Giardia exhibits a biphasic lifestyle existing as either a dormant cyst or a vegetative trophozoite. Infections are typically initiated through the consumption of cyst-contaminated water or food. Giardia was first axenized in the 1970s and can be readily maintained in a laboratory setting. Additionally, Giardia is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain Giardia and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. © 2020 Wiley Periodicals LLC.

Basic Protocol 1: In vitro maintenance and growth of Giardia trophozoites

Basic Protocol 2: In vitro encystation of Giardia cysts

Basic Protocol 3: In vivo infections using Giardia trophozoites

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

自引率

0.00%

发文量

期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.

期刊最新文献

Issue Information Programmable Gene Knockdown in Diverse Bacteria Using Mobile-CRISPRi Gene Editing in Dimorphic Fungi Using CRISPR/Cas9 Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors 3D Oral and Cervical Tissue Models for Studying Papillomavirus Host-Pathogen Interactions