β-胡萝卜素抑制NF-κB及抑制二乙基亚硝胺致Wistar大鼠肝脏炎症。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS International Journal for Vitamin and Nutrition Research Pub Date : 2022-10-01 Epub Date: 2020-07-20 DOI:10.1024/0300-9831/a000665
Uzma Latief, Riaz Ahmad
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引用次数: 5

摘要

β-胡萝卜素通过多种生化机制表现出抗氧化和保护肝脏的活性。然而,这种胡萝卜素在慢性肝病中的抗氧化和抗炎作用机制尚不完全清楚。在目前的研究中,我们试图概述β-胡萝卜素对白化Wistar大鼠肝纤维化的作用机制。采用二乙基亚硝胺(DEN)诱导大鼠肝纤维化2周。将DEN处理大鼠分为4组,每组5只。体内补充β-胡萝卜素可通过抑制iNOS和NF-κB来减弱den诱导的LFT标志物(P)升高。因此,β-胡萝卜素可能被建议作为一种可能的营养品来抑制实验性肝纤维化。
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β-Carotene inhibits NF-κB and restrains diethylnitrosamine-induced hepatic inflammation in Wistar rats.

β-Carotene exhibits antioxidant and hepatoprotective activities via a multitude of biochemical mechanisms. However, the action mechanism involved in antioxidant and anti-inflammatory effects of this carotene in chronic liver diseases is not fully understood. In the present investigation, we have attempted to outline a plausible mechanism of β-carotene action against liver fibrosis in albino Wistar rats. To induce hepatic fibrosis, diethylnitrosamine (DEN) was administered in experimental rats for two weeks. DEN treated rats were divided into four groups, wherein each group comprised of five rats. β-Carotene supplement attenuated DEN-induced elevation in LFT markers (P < 0.05); averted depletion of glycogen (24%, P < 0.05) and, increased nitrite (P < 0.05), hydroxyproline (~67%, P < 0.05) and collagen levels (~65%, P < 0.05). Confocal microscopy of tissue sections stained with picrosirius red revealed accrued collagen in DEN-administered group, which was found to be reduced by β-carotene supplementation. Furthermore, β-carotene decreased the expression of iNOS/NOS-2 and NF-κB, as revealed by immunohistochemistry and Western immunoblotting. Collectively, these results demonstrate that β-carotene mitigates experimental liver fibrosis via inhibition of iNOS and NF-κB in-vivo. Thus, β-carotene may be suggested as a possible nutraceutical to curb experimental liver fibrosis.

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来源期刊
CiteScore
5.00
自引率
4.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: Since 1930 this journal has provided an important international forum for scientific advances in the study of nutrition and vitamins. Widely read by academicians as well as scientists working in major governmental and corporate laboratories throughout the world, this publication presents work dealing with basic as well as applied topics in the field of micronutrients, macronutrients, and non-nutrients such as secondary plant compounds. The editorial and advisory boards include many of the leading persons currently working in this area. The journal is of particular interest to: - Nutritionists - Vitaminologists - Biochemists - Physicians - Engineers of human and animal nutrition - Food scientists
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