产前铅暴露和脐带血DNA甲基化进展:一项全表观基因组关联研究。

IF 4.8 Q1 GENETICS & HEREDITY Environmental Epigenetics Pub Date : 2020-12-08 eCollection Date: 2020-01-01 DOI:10.1093/eep/dvaa014
Jonathan A Heiss, Martha M Téllez-Rojo, Guadalupe Estrada-Gutiérrez, Lourdes Schnaas, Chitra Amarasiriwardena, Andrea A Baccarelli, Robert O Wright, Allan C Just
{"title":"产前铅暴露和脐带血DNA甲基化进展:一项全表观基因组关联研究。","authors":"Jonathan A Heiss,&nbsp;Martha M Téllez-Rojo,&nbsp;Guadalupe Estrada-Gutiérrez,&nbsp;Lourdes Schnaas,&nbsp;Chitra Amarasiriwardena,&nbsp;Andrea A Baccarelli,&nbsp;Robert O Wright,&nbsp;Allan C Just","doi":"10.1093/eep/dvaa014","DOIUrl":null,"url":null,"abstract":"<p><p>The effects of prenatal lead exposure on child development include impaired growth and cognitive function. DNA methylation might be involved in the underlying mechanisms and previous epigenome-wide association studies reported associations between lead exposure during pregnancy and cord blood methylation levels. However, it is unclear during which developmental stage lead exposure is most harmful. Cord blood methylation levels were assayed in 420 children from a Mexican pre-birth cohort using the Illumina Infinium MethylationEPIC microarray. Lead concentrations were measured in umbilical cord blood as well as in blood samples from the mothers collected at 2nd and 3rd trimester and delivery using inductively coupled plasma-mass spectrometry. In addition, maternal bone lead levels were measured in tibia and patella using X-ray fluorescence. Comprehensive quality control and preprocessing of microarray data was followed by an unbiased restriction to methylation sites with substantial variance. Methylation levels at 202 111 cytosine-phosphate-guanine sites were regressed on each exposure adjusting for child sex, leukocyte composition, batch variables, gestational age, birthweight-for-gestational-age, maternal age, maternal education and mode of delivery. We find no association between prenatal lead exposure and cord blood methylation. This null result is strengthened by a sensitivity analysis showing that in the same dataset known biomarkers for birthweight-for-gestational-age can be recovered and the fact that phenotypic associations with lead exposure have been described in the same cohort.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvaa014","citationCount":"8","resultStr":"{\"title\":\"Prenatal lead exposure and cord blood DNA methylation in PROGRESS: an epigenome-wide association study.\",\"authors\":\"Jonathan A Heiss,&nbsp;Martha M Téllez-Rojo,&nbsp;Guadalupe Estrada-Gutiérrez,&nbsp;Lourdes Schnaas,&nbsp;Chitra Amarasiriwardena,&nbsp;Andrea A Baccarelli,&nbsp;Robert O Wright,&nbsp;Allan C Just\",\"doi\":\"10.1093/eep/dvaa014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The effects of prenatal lead exposure on child development include impaired growth and cognitive function. DNA methylation might be involved in the underlying mechanisms and previous epigenome-wide association studies reported associations between lead exposure during pregnancy and cord blood methylation levels. However, it is unclear during which developmental stage lead exposure is most harmful. Cord blood methylation levels were assayed in 420 children from a Mexican pre-birth cohort using the Illumina Infinium MethylationEPIC microarray. Lead concentrations were measured in umbilical cord blood as well as in blood samples from the mothers collected at 2nd and 3rd trimester and delivery using inductively coupled plasma-mass spectrometry. In addition, maternal bone lead levels were measured in tibia and patella using X-ray fluorescence. Comprehensive quality control and preprocessing of microarray data was followed by an unbiased restriction to methylation sites with substantial variance. Methylation levels at 202 111 cytosine-phosphate-guanine sites were regressed on each exposure adjusting for child sex, leukocyte composition, batch variables, gestational age, birthweight-for-gestational-age, maternal age, maternal education and mode of delivery. We find no association between prenatal lead exposure and cord blood methylation. This null result is strengthened by a sensitivity analysis showing that in the same dataset known biomarkers for birthweight-for-gestational-age can be recovered and the fact that phenotypic associations with lead exposure have been described in the same cohort.</p>\",\"PeriodicalId\":11774,\"journal\":{\"name\":\"Environmental Epigenetics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2020-12-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1093/eep/dvaa014\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Epigenetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/eep/dvaa014\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Epigenetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/eep/dvaa014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 8

摘要

产前铅暴露对儿童发育的影响包括生长和认知功能受损。DNA甲基化可能涉及潜在的机制,之前的全表观基因组关联研究报告了妊娠期间铅暴露与脐带血甲基化水平之间的关联。然而,尚不清楚在哪个发育阶段铅暴露是最有害的。使用Illumina Infinium MethylationEPIC微阵列检测来自墨西哥产前队列的420名儿童的脐带血甲基化水平。使用电感耦合血浆质谱法测量脐带血中的铅浓度以及在妊娠第2和第3个月和分娩时收集的母亲的血液样本。此外,利用x射线荧光测定了产妇胫骨和髌骨的骨铅水平。对微阵列数据进行全面的质量控制和预处理,然后对存在较大差异的甲基化位点进行无偏限制。在每次暴露后,对202 - 111个胞嘧啶-磷酸-鸟嘌呤位点的甲基化水平进行回归,调整了儿童性别、白细胞组成、批次变量、胎龄、出生体重/胎龄、母亲年龄、母亲教育程度和分娩方式。我们没有发现产前铅暴露和脐带血甲基化之间的联系。敏感性分析表明,在同一数据集中可以恢复已知的出生体重与胎龄的生物标志物,并且在同一队列中描述了与铅暴露的表型关联,从而加强了这一无效结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Prenatal lead exposure and cord blood DNA methylation in PROGRESS: an epigenome-wide association study.

The effects of prenatal lead exposure on child development include impaired growth and cognitive function. DNA methylation might be involved in the underlying mechanisms and previous epigenome-wide association studies reported associations between lead exposure during pregnancy and cord blood methylation levels. However, it is unclear during which developmental stage lead exposure is most harmful. Cord blood methylation levels were assayed in 420 children from a Mexican pre-birth cohort using the Illumina Infinium MethylationEPIC microarray. Lead concentrations were measured in umbilical cord blood as well as in blood samples from the mothers collected at 2nd and 3rd trimester and delivery using inductively coupled plasma-mass spectrometry. In addition, maternal bone lead levels were measured in tibia and patella using X-ray fluorescence. Comprehensive quality control and preprocessing of microarray data was followed by an unbiased restriction to methylation sites with substantial variance. Methylation levels at 202 111 cytosine-phosphate-guanine sites were regressed on each exposure adjusting for child sex, leukocyte composition, batch variables, gestational age, birthweight-for-gestational-age, maternal age, maternal education and mode of delivery. We find no association between prenatal lead exposure and cord blood methylation. This null result is strengthened by a sensitivity analysis showing that in the same dataset known biomarkers for birthweight-for-gestational-age can be recovered and the fact that phenotypic associations with lead exposure have been described in the same cohort.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Environmental Epigenetics
Environmental Epigenetics GENETICS & HEREDITY-
CiteScore
6.50
自引率
5.30%
发文量
0
审稿时长
17 weeks
期刊最新文献
Correction to: To live or let die? Epigenetic adaptations to climate change-a review. Bronchial cell epigenetic aging in a human experimental study of short-term diesel and ozone exposures. Epigenetic transgenerational inheritance of toxicant exposure-specific non-coding RNA in sperm. Environmental conditions elicit a slow but enduring response of histone post-translational modifications in Mozambique tilapia. Impaired energy expenditure following exposure to either DDT or DDE in mice may be mediated by DNA methylation changes in brown adipose.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1