Steven M. Valles , Jason B. Oliver , Karla M. Addesso , Omaththage P. Perera
{"title":"红火蚁与红火蚁杂交火蚁的独特毒液蛋白","authors":"Steven M. Valles , Jason B. Oliver , Karla M. Addesso , Omaththage P. Perera","doi":"10.1016/j.toxcx.2021.100065","DOIUrl":null,"url":null,"abstract":"<div><p>The Solenopsis venom protein 2 transcript was amplified, sequenced, probed, and analyzed from <em>Solenopsis invicta</em> x <em>Solenopsis richteri</em> hybrid ant colonies (hybrids) collected from across Tennessee to determine the extent of introgression of each parent allele (<em>Solenopsis invicta</em> venom protein 2 [Soli2] and Solenopsis richteri venom protein 2 [Solr2]). Chemotaxonomic analyses of venom alkaloids and cuticular hydrocarbons were used to categorize hybrid colonies and their relative relatedness to each parent species. Hybrid colonies were chosen randomly from each chemotaxonomic hybridization category, including “very near <em>S. richteri</em>,” “near <em>S. richteri</em>,” “near <em>S. invicta</em>,” and “very near <em>S. invicta</em>.” Lateral flow immunoassays for detection of the Soli2 and Solr2 venom proteins were largely in agreement with the chemotaxonomic analyses for the very near <em>S. richteri</em> (100% Solr2) and very near <em>S. invicta</em> (80% Soli2, 20% Soli2 + Solr2 detected in the sample) groups, while Soli2 and Solr2 were reported in 60% and 40% in the near <em>S. invicta</em> and near <em>S. richteri</em> chemotaxonomic groups. Analysis of transcripts from the hybrid colonies revealed a sequence with 100% identity to Soli2 (GenBank Accession L09560) and three unique sequences, which we identify as Solenopsis hybrid venom protein 2 (Solh2; GenBank Accession MT150127), Solenopsis hybrid truncated venom protein 2 (Solh2<sup>Tr97</sup>; Genbank Accession MT150129), and Solenopsis richteri venom protein 2, D to A change at position 69 (Solr2<sup>A69</sup>; GenBank Accession MT150128). The predicted open reading frame for Solh2 and Solh2<sup>Tr97</sup> revealed sequences unique to hybrid ants, with Solh2<sup>Tr97</sup>an alternatively spliced form. A third unique sequence, Solr2<sup>A69</sup>, is likely the correct sequence for Solr2, which appears to have been published previously with a sequencing error (GenBank Accession P35776).</p></div>","PeriodicalId":37124,"journal":{"name":"Toxicon: X","volume":"9 ","pages":"Article 100065"},"PeriodicalIF":3.6000,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.toxcx.2021.100065","citationCount":"1","resultStr":"{\"title\":\"Unique venom proteins from Solenopsis invicta x Solenopsis richteri hybrid fire ants\",\"authors\":\"Steven M. Valles , Jason B. Oliver , Karla M. Addesso , Omaththage P. Perera\",\"doi\":\"10.1016/j.toxcx.2021.100065\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The Solenopsis venom protein 2 transcript was amplified, sequenced, probed, and analyzed from <em>Solenopsis invicta</em> x <em>Solenopsis richteri</em> hybrid ant colonies (hybrids) collected from across Tennessee to determine the extent of introgression of each parent allele (<em>Solenopsis invicta</em> venom protein 2 [Soli2] and Solenopsis richteri venom protein 2 [Solr2]). Chemotaxonomic analyses of venom alkaloids and cuticular hydrocarbons were used to categorize hybrid colonies and their relative relatedness to each parent species. Hybrid colonies were chosen randomly from each chemotaxonomic hybridization category, including “very near <em>S. richteri</em>,” “near <em>S. richteri</em>,” “near <em>S. invicta</em>,” and “very near <em>S. invicta</em>.” Lateral flow immunoassays for detection of the Soli2 and Solr2 venom proteins were largely in agreement with the chemotaxonomic analyses for the very near <em>S. richteri</em> (100% Solr2) and very near <em>S. invicta</em> (80% Soli2, 20% Soli2 + Solr2 detected in the sample) groups, while Soli2 and Solr2 were reported in 60% and 40% in the near <em>S. invicta</em> and near <em>S. richteri</em> chemotaxonomic groups. Analysis of transcripts from the hybrid colonies revealed a sequence with 100% identity to Soli2 (GenBank Accession L09560) and three unique sequences, which we identify as Solenopsis hybrid venom protein 2 (Solh2; GenBank Accession MT150127), Solenopsis hybrid truncated venom protein 2 (Solh2<sup>Tr97</sup>; Genbank Accession MT150129), and Solenopsis richteri venom protein 2, D to A change at position 69 (Solr2<sup>A69</sup>; GenBank Accession MT150128). The predicted open reading frame for Solh2 and Solh2<sup>Tr97</sup> revealed sequences unique to hybrid ants, with Solh2<sup>Tr97</sup>an alternatively spliced form. A third unique sequence, Solr2<sup>A69</sup>, is likely the correct sequence for Solr2, which appears to have been published previously with a sequencing error (GenBank Accession P35776).</p></div>\",\"PeriodicalId\":37124,\"journal\":{\"name\":\"Toxicon: X\",\"volume\":\"9 \",\"pages\":\"Article 100065\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2021-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.toxcx.2021.100065\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxicon: X\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590171021000011\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicon: X","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590171021000011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 1
摘要
从田纳西州收集的Solenopsis invicta和Solenopsis richteri杂交蚁群(杂交蚁群)中扩增、测序、探测和分析了Solenopsis venom protein 2转录本,以确定每个亲本等位基因(Solenopsis invicta venom protein 2 [Soli2]和Solenopsis richteri venom protein 2 [Solr2])的基因导入程度。利用毒液生物碱和表皮碳氢化合物的化学分类分析对杂交菌落进行了分类,并对其与各亲本种的相对亲缘关系进行了分析。从每个化学分类杂交类别中随机选择杂交菌落,包括“非常接近S. richteri”,“接近S. richteri”,“接近S. invicta”和“非常接近S. invicta”。检测Soli2和Solr2毒液蛋白的横向流动免疫分析与非常接近李氏弧菌(100% Solr2)和非常接近invicta(样品中检测到80% Solr2, 20% Soli2 + Solr2)组的化学分类分析基本一致,而在接近invicta和接近李氏弧菌的化学分类组中,Soli2和Solr2分别为60%和40%。对杂交菌落的转录本进行分析,发现一个序列与Soli2 (GenBank登录L09560) 100%同源,并有三个独特的序列,我们将其鉴定为Solenopsis hybrid venom protein 2 (Solh2;GenBank Accession MT150127), Solenopsis hybrid truncated venom protein 2 (Solh2Tr97;Genbank加入MT150129),索理梭菌毒液蛋白2,D到A在第69位发生变化(Solr2A69;GenBank Accession MT150128)。预测的Solh2和Solh2Tr97的开放阅读框显示了杂交蚂蚁特有的序列,其中Solh2Tr97是一种选择性拼接形式。第三个独特的序列,Solr2A69,可能是Solr2的正确序列,该序列似乎在之前发表过测序错误(GenBank登录P35776)。
Unique venom proteins from Solenopsis invicta x Solenopsis richteri hybrid fire ants
The Solenopsis venom protein 2 transcript was amplified, sequenced, probed, and analyzed from Solenopsis invicta x Solenopsis richteri hybrid ant colonies (hybrids) collected from across Tennessee to determine the extent of introgression of each parent allele (Solenopsis invicta venom protein 2 [Soli2] and Solenopsis richteri venom protein 2 [Solr2]). Chemotaxonomic analyses of venom alkaloids and cuticular hydrocarbons were used to categorize hybrid colonies and their relative relatedness to each parent species. Hybrid colonies were chosen randomly from each chemotaxonomic hybridization category, including “very near S. richteri,” “near S. richteri,” “near S. invicta,” and “very near S. invicta.” Lateral flow immunoassays for detection of the Soli2 and Solr2 venom proteins were largely in agreement with the chemotaxonomic analyses for the very near S. richteri (100% Solr2) and very near S. invicta (80% Soli2, 20% Soli2 + Solr2 detected in the sample) groups, while Soli2 and Solr2 were reported in 60% and 40% in the near S. invicta and near S. richteri chemotaxonomic groups. Analysis of transcripts from the hybrid colonies revealed a sequence with 100% identity to Soli2 (GenBank Accession L09560) and three unique sequences, which we identify as Solenopsis hybrid venom protein 2 (Solh2; GenBank Accession MT150127), Solenopsis hybrid truncated venom protein 2 (Solh2Tr97; Genbank Accession MT150129), and Solenopsis richteri venom protein 2, D to A change at position 69 (Solr2A69; GenBank Accession MT150128). The predicted open reading frame for Solh2 and Solh2Tr97 revealed sequences unique to hybrid ants, with Solh2Tr97an alternatively spliced form. A third unique sequence, Solr2A69, is likely the correct sequence for Solr2, which appears to have been published previously with a sequencing error (GenBank Accession P35776).