NR4A3 (NOR-1)免疫染色在涎腺腺泡细胞癌中的表现优于DOG1免疫染色的初步研究

IF 1 Q3 DENTISTRY, ORAL SURGERY & MEDICINE eJournal of Oral Maxillofacial Research Pub Date : 2021-03-31 eCollection Date: 2021-01-01 DOI:10.5037/jomr.2021.12104
Adepitan Owosho, Donald Tyler, Olufunlola Adesina, Oluwole Odujoko, Kurt Summersgill
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引用次数: 8

摘要

目的:唾液腺腺泡细胞癌具有复发性和特异性染色体重排[t(4;9)(q13;q31)],导致分泌性钙结合磷酸化蛋白基因簇在4q13位易位至9q31位核受体亚家族4组成员3。这可以通过免疫组织化学检测到转录因子核受体亚家族4 A组成员3的上调。本初步研究的目的是评估核受体亚家族4组A成员3免疫染色在全片腺泡细胞癌组织上的表现,并与GIST-1免疫染色发现的结果进行比较。材料和方法:我们收集了6例腺泡细胞癌(AciCC),其中5例为常规低级别癌,1例为去分化高级别癌。在宾夕法尼亚州匹兹堡的匹兹堡大学医学中心对所有检索到的病例进行核受体亚家族4组A成员3 (NR4A3)和GIST-1 (DOG1)的免疫组化(IHC)。结果:NR4A3细胞免疫组化优于DOG1细胞免疫组化:6例AciCC(含去分化高级别)中有5例(83.3%)表现为NR4A3强弥漫核染色,5例AciCC(含去分化高级别)表现为弱至中度膜性染色,DOG1分布不均匀。此外,只有3例(50%)患者显示完全的膜性染色。结论:本初步研究表明核受体亚家族4组A成员3免疫染色是免疫细胞癌的敏感标志物,在诊断免疫细胞癌方面比GIST-1免疫染色有更好的应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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NR4A3 (NOR-1) Immunostaining Shows Better Performance than DOG1 Immunostaining in Acinic Cell Carcinoma of Salivary Gland: a Preliminary Study.

Objectives: Acinic cell carcinoma of salivary gland harbours recurrent and specific chromosomal rearrangement [t(4;9)(q13;q31)], resulting in the translocation of secretory calcium-binding phosphoprotein gene cluster at 4q13 to nuclear receptor subfamily 4 group a member 3 at 9q31. This upregulates the transcription factor nuclear receptor subfamily 4 group A member 3, which can be detected by immunohistochemistry. The purpose of this pilot study is to evaluate the performance of nuclear receptor subfamily 4 group A member 3 immunostaining on whole-slide acinic cell carcinoma tissue, in comparison with discovered on GIST-1 immunostaining.

Material and methods: We retrieved 6 cases of acinic cell carcinoma (AciCC), including 5 conventional low-grade and 1 dedifferentiated high-grade. Immunohistochemistry (IHC) for nuclear receptor subfamily 4 group A member 3 (NR4A3) and discovered on GIST-1 (DOG1) were performed at the University of Pittsburgh Medical Centre in Pittsburgh, Pennsylvania on all retrieved cases.

Results: The result shows that NR4A3 IHC shows better performance than DOG1 IHC: 5 of the 6 (83.3%) AciCC cases (including the dedifferentiated high-grade) demonstrated strong diffuse nuclear staining for NR4A3, also five AciCC cases (including the dedifferentiated high-grade) demonstrated weak to moderate membranous staining with variable distribution for DOG1. Moreover, only 3 (50%) cases showed complete membranous staining with DOG1.

Conclusions: This pilot study showed that nuclear receptor subfamily 4 group A member 3 immunostaining is a sensitive marker for acinic cell carcinoma and of better utility than discovered on GIST-1 immunostaining in making a diagnosis of acinic cell carcinoma.

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