Sofia Maraki , Dimitra Stafylaki , Spyridon A. Karageorgos , Filippos Koutroumpakis , George Hamilos , Antonis Makrigiannakis , George Samonis
{"title":"羊水对不同念珠菌的抑菌活性研究","authors":"Sofia Maraki , Dimitra Stafylaki , Spyridon A. Karageorgos , Filippos Koutroumpakis , George Hamilos , Antonis Makrigiannakis , George Samonis","doi":"10.1016/j.riam.2021.02.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Although <em>Candida</em> is a commensal of the urogenital tract, intrauterine fungal infections are extremely uncommon in clinical practice.</p></div><div><h3>Aims</h3><p>In the present work we evaluated whether amniotic fluid (AF) possesses direct antifungal activity against clinical isolates of <em>Candida</em> <em>albicans</em> and other <em>Candida</em> species.</p></div><div><h3>Methods</h3><p>A total of 23 AF samples from pregnant women with gestational age of 38–41 weeks were obtained under aseptic conditions by the aspiration of the amniotic sac during cesarean section. Different <em>Candida</em> species were inoculated in amniotic fluid and Sabouraud broth, used as control, and were incubated at 37<!--> <!-->°C for 48<!--> <!-->h. Quantitative cultures of test samples and controls were performed at 0, 4, 8, 12, 24, and 48<!--> <!-->h.</p></div><div><h3>Results</h3><p>AF collected from 23 pregnant women had consistent and significant inhibitory activity against all <em>Candida</em> isolates tested. Nonetheless, a complete inhibition of growth by all 23 AF samples tested was observed only against <span><em>Candida glabrata</em></span>.</p></div><div><h3>Conclusions</h3><p>It is likely that the antifungal activity of the AF against <em>C. albicans</em>, <em>C. glabrata</em> and <span><em>Candida parapsilosis</em></span> observed <em>in vitro</em> also exists <em>in vivo</em>, contributing to protect against intrauterine fungal infections.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 148-150"},"PeriodicalIF":1.5000,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.02.002","citationCount":"0","resultStr":"{\"title\":\"Antifungal activity of amniotic fluid against different Candida species\",\"authors\":\"Sofia Maraki , Dimitra Stafylaki , Spyridon A. Karageorgos , Filippos Koutroumpakis , George Hamilos , Antonis Makrigiannakis , George Samonis\",\"doi\":\"10.1016/j.riam.2021.02.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Although <em>Candida</em> is a commensal of the urogenital tract, intrauterine fungal infections are extremely uncommon in clinical practice.</p></div><div><h3>Aims</h3><p>In the present work we evaluated whether amniotic fluid (AF) possesses direct antifungal activity against clinical isolates of <em>Candida</em> <em>albicans</em> and other <em>Candida</em> species.</p></div><div><h3>Methods</h3><p>A total of 23 AF samples from pregnant women with gestational age of 38–41 weeks were obtained under aseptic conditions by the aspiration of the amniotic sac during cesarean section. Different <em>Candida</em> species were inoculated in amniotic fluid and Sabouraud broth, used as control, and were incubated at 37<!--> <!-->°C for 48<!--> <!-->h. Quantitative cultures of test samples and controls were performed at 0, 4, 8, 12, 24, and 48<!--> <!-->h.</p></div><div><h3>Results</h3><p>AF collected from 23 pregnant women had consistent and significant inhibitory activity against all <em>Candida</em> isolates tested. Nonetheless, a complete inhibition of growth by all 23 AF samples tested was observed only against <span><em>Candida glabrata</em></span>.</p></div><div><h3>Conclusions</h3><p>It is likely that the antifungal activity of the AF against <em>C. albicans</em>, <em>C. glabrata</em> and <span><em>Candida parapsilosis</em></span> observed <em>in vitro</em> also exists <em>in vivo</em>, contributing to protect against intrauterine fungal infections.</p></div>\",\"PeriodicalId\":21291,\"journal\":{\"name\":\"Revista Iberoamericana De Micologia\",\"volume\":\"38 3\",\"pages\":\"Pages 148-150\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2021-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.riam.2021.02.002\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Iberoamericana De Micologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S113014062100019X\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S113014062100019X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
Antifungal activity of amniotic fluid against different Candida species
Background
Although Candida is a commensal of the urogenital tract, intrauterine fungal infections are extremely uncommon in clinical practice.
Aims
In the present work we evaluated whether amniotic fluid (AF) possesses direct antifungal activity against clinical isolates of Candidaalbicans and other Candida species.
Methods
A total of 23 AF samples from pregnant women with gestational age of 38–41 weeks were obtained under aseptic conditions by the aspiration of the amniotic sac during cesarean section. Different Candida species were inoculated in amniotic fluid and Sabouraud broth, used as control, and were incubated at 37 °C for 48 h. Quantitative cultures of test samples and controls were performed at 0, 4, 8, 12, 24, and 48 h.
Results
AF collected from 23 pregnant women had consistent and significant inhibitory activity against all Candida isolates tested. Nonetheless, a complete inhibition of growth by all 23 AF samples tested was observed only against Candida glabrata.
Conclusions
It is likely that the antifungal activity of the AF against C. albicans, C. glabrata and Candida parapsilosis observed in vitro also exists in vivo, contributing to protect against intrauterine fungal infections.
期刊介绍：
Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.
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