Matrigel®增强3T3-L1细胞分化。

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Adipocyte Pub Date : 2021-12-01 DOI:10.1080/21623945.2021.1951985
Chitmandeep Josan, Sachin Kakar, Sandeep Raha
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引用次数: 5

摘要

在生物凝胶上培养细胞被认为提供了更像体内的细胞外基质。与在组织培养物包被的聚苯乙烯表面上生长相比,在Matrigel®上培养的3T3-L1细胞显著改变了其增殖和分化。在250μm厚的Matrigel®层上生长有助于形成3T3-L1细胞的细胞聚集体。与直接在聚苯乙烯组织培养表面上培养的细胞相比,在Matrigel®上培养的3T3-L1细胞的分化显示关键脂肪生成转录因子(PPARγ、C/EBPα、SREBP1)、脂肪生成标记物(FAS、FABP4、LPL、PLIN1)和脂肪细胞成熟标记物(LEP)的mRNA水平增加。在Matrigel®培养的3T3-L1细胞中,细胞外基质蛋白(FN1、COL1A1、COL4A1、COL6、LAM)的基因表达降低。此外,在存在和不存在罗格列酮的情况下,Matrigel®上的生长增加了3T3-L1细胞中的脂质积累,罗格列酮是一种噻唑烷二酮,通常用于优化这些细胞的分化。脂肪细胞基因表达和脂质积累模式的这些变化可能是Matrigel®上发生的细胞-细胞和细胞-ECM相互作用增加的结果,这种情况更能反映体内模型。总之,我们的数据促进了对在Matrigel®上培养3T3-L1细胞的价值的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Matrigel® enhances 3T3-L1 cell differentiation.

Culturing cells on bio-gels are believed to provide a more in vivo-like extracellular matrix. 3T3-L1 cells cultured on Matrigel® significantly alteregd their proliferation and differentiation as compared to growth on tissue culture-coated polystyrene surfaces. Growth on a 250-μm thick layer of Matrigel® facilitated the formation of cellular aggregates of 3T3-L1 cells. Differentiation of 3T3-L1 cells cultured on Matrigel® demonstrated increased levels of mRNA levels for key adipogenic transcription factors (PPARγ, C/EBPα, SREBP1), lipogenic markers (FAS, FABP4, LPL, PLIN1) and markers of adipocyte maturity (LEP), compared to cells cultured directly on a polystyrene tissue culture surface. The gene expression of extracellular matrix proteins (FN1, COL1A1, COL4A1, COL6, LAM) was decreased in 3T3-L1 cells cultured on Matrigel®. Furthermore, growth on Matrigel® increased lipid accumulation in 3T3-L1 cells in the presence and absence of rosiglitazone, a thiazolidinedione routinely used to optimize differentiation in these cells. These changes in adipocyte gene expression and lipid accumulation patterns may be a result of the increased cell-cell and cell-ECM interactions occurring on the Matrigel®, a scenario that is more reflective of an in vivo model. Taken together, our data advance the understanding of the value of culturing 3T3-L1 cells on Matrigel®.

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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
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