两株乳酸乳球菌亚种的基因组序列分析。祖先相同但产生外多糖的能力不同的Cremoris。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-30 DOI:10.2323/jgam.2021.03.001
Yayoi Gotoh, Kyosuke Kita, Kosei Tanaka, Shu Ishikawa, Toshio Suzuki, Ken-Ichi Yoshida
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引用次数: 1

摘要

乳酸乳球菌亚种的研究。Cremoris用于生产含有粘性外多糖的酸奶。将产胞外多糖的菌株G3-2在高温下培养,分离出失去胞外多糖生物合成能力的自发突变体EPSC。两株菌株的基因组均由一条2.4 mb的染色体和多达11个质粒组成,其中一个编码胞外多糖生物合成基因簇的质粒在EPSC中选择性丢失。
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Genome sequences of two strains of Lactococcus lactis subsp. cremoris with the same ancestry but a different capacity to produce exopolysaccharides.

Strains of Lactococcus lactis subsp. cremoris are used to produce yogurt containing exopolysaccharides with a sticky texture. When strain G3-2 producing exopolysaccharides was grown at elevated temperatures, a spontaneous mutant EPSC, which had lost exopolysaccharides biosynthesis, was isolated. Genomes of the two strains were determined to be composed of a 2.4-Mb chromosome and up to eleven plasmids, and it was revealed that one of the plasmids encoding the gene cluster for exopolysaccharides biosynthesis was lost selectively in EPSC.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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