{"title":"具有抗真菌活性的阿霉素链霉菌GH家族20 Exo-β- n -乙酰己糖氨酸酶的鉴定","authors":"Naoki Shirasaka, Koichi Harazono, Ryota Nakahigashi, Keigo Mitsui, Jun Tanaka, Sayaka Tanazawa, Masaru Mitsutomi, Takayuki Ohnuma","doi":"10.5458/jag.jag.JAG-2019_0001","DOIUrl":null,"url":null,"abstract":"<p><p>We characterized <i>Sa</i>HEX, which is a glycoside hydrolase (GH) family 20 exo-β-<i>N</i>-acetylhexosaminidase found in <i>Streptomyces avermitilis</i>. <i>Sa</i>HEX exolytically hydrolyzed chitin oligosaccharides from their non-reducing ends, and yielded <i>N</i>-acetylglucosamine (GlcNAc) as the end product. According to the initial rate of substrate hydrolysis, the rates of (GlcNAc)<sub>3</sub> and (GlcNAc)<sub>5</sub> hydrolysis were greater than the rates for the other oligosaccharides. The enzyme exhibited antifungal activity against <i>Aspergillus niger</i>, which was probably due to hydrolytic activity with regard to chitin in the hyphal tips. Therefore, <i>Sa</i>HEX has potential for use in GlcNAc production and food preservation.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2019-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5458/jag.jag.JAG-2019_0001","citationCount":"1","resultStr":"{\"title\":\"Characterization of a GH Family 20 Exo-β-<i>N</i>-acetylhexosaminidase with Antifungal Activity from <i>Streptomyces avermitilis</i>.\",\"authors\":\"Naoki Shirasaka, Koichi Harazono, Ryota Nakahigashi, Keigo Mitsui, Jun Tanaka, Sayaka Tanazawa, Masaru Mitsutomi, Takayuki Ohnuma\",\"doi\":\"10.5458/jag.jag.JAG-2019_0001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We characterized <i>Sa</i>HEX, which is a glycoside hydrolase (GH) family 20 exo-β-<i>N</i>-acetylhexosaminidase found in <i>Streptomyces avermitilis</i>. <i>Sa</i>HEX exolytically hydrolyzed chitin oligosaccharides from their non-reducing ends, and yielded <i>N</i>-acetylglucosamine (GlcNAc) as the end product. According to the initial rate of substrate hydrolysis, the rates of (GlcNAc)<sub>3</sub> and (GlcNAc)<sub>5</sub> hydrolysis were greater than the rates for the other oligosaccharides. The enzyme exhibited antifungal activity against <i>Aspergillus niger</i>, which was probably due to hydrolytic activity with regard to chitin in the hyphal tips. Therefore, <i>Sa</i>HEX has potential for use in GlcNAc production and food preservation.</p>\",\"PeriodicalId\":14999,\"journal\":{\"name\":\"Journal of applied glycoscience\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2019-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.5458/jag.jag.JAG-2019_0001\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of applied glycoscience\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5458/jag.jag.JAG-2019_0001\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2019/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of applied glycoscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5458/jag.jag.JAG-2019_0001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 1
摘要
我们鉴定了SaHEX，这是一种在阿维链霉菌中发现的糖苷水解酶(GH)家族20外显子-β- n -乙酰己糖氨酸酶。SaHEX从其非还原端外解水解甲壳素寡糖，并产生n-乙酰氨基葡萄糖(GlcNAc)作为最终产物。根据底物水解的初始速率，(GlcNAc)3和(GlcNAc)5的水解速率大于其他低聚糖的水解速率。该酶对黑曲霉表现出抗真菌活性，这可能是由于其对菌丝尖端的几丁质具有水解活性。因此，SaHEX在GlcNAc生产和食品保鲜方面具有潜力。
Characterization of a GH Family 20 Exo-β-N-acetylhexosaminidase with Antifungal Activity from Streptomyces avermitilis.
We characterized SaHEX, which is a glycoside hydrolase (GH) family 20 exo-β-N-acetylhexosaminidase found in Streptomyces avermitilis. SaHEX exolytically hydrolyzed chitin oligosaccharides from their non-reducing ends, and yielded N-acetylglucosamine (GlcNAc) as the end product. According to the initial rate of substrate hydrolysis, the rates of (GlcNAc)3 and (GlcNAc)5 hydrolysis were greater than the rates for the other oligosaccharides. The enzyme exhibited antifungal activity against Aspergillus niger, which was probably due to hydrolytic activity with regard to chitin in the hyphal tips. Therefore, SaHEX has potential for use in GlcNAc production and food preservation.
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