韩国黑壳居群地理遗传差异分析。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI:10.12717/DR.2021.25.2.105
Jong-Man Yoon
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引用次数: 1

摘要

利用pcr建立的遗传分析目标和原理,建立了2个方贝居群Scapharca broughtonii (YEOSU,丽水居群和JINHAE,镇海居群)欧几里得遗传距离(GDs)的等级极枝图。利用5条寡核苷酸引物,在丽水和镇海种群中分别构建了354条和390条可分带,DNA片段大小从100 bp扩展到1600 bp。bandsharing (BS)结果显示,镇海人口的平均BS值(0.700)高于丽水人口(0.692)。个体之间的GD支持II组的相邻关联(JINHAE 12 - JINHAE 22)。对两个Scapharca种群之间显著GD的观察证实,这种pcr生成的技术可能是一种有益的尝试,用于种群内部和种群之间的生物DNA审查。PCR调查的潜力将有利于在水产养殖生产中选择一些与繁殖和/或检疫相关的性状的个体和/或群体。
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Analysis of Geographical Genetic Differences of Arkshell Populations in Korea.

A PCR-founded genetic analysis aim and principle was used to foster a hierarchical polar dendrogram of the Euclidean genetic distances (GDs) for two arkshell populations, Scapharca broughtonii (YEOSU, Yeosu population and JINHAE, Jinhae population). Five oligonucleotides primers were make use of to craft 354 and 390 scorable bands in the Yeosu and Jinhae populations, respectively, outspreading in DNA fragment size from 100 bp to 1,600 bp. The bandsharing (BS) results disclosed that the Jinhae population had a higher average BS value (0.700) than that for the Yeosu population (0.692). The GD between individuals supported an adjacent association in grouping II (JINHAE 12 - JINHAE 22). The observation of a noteworthy GD between the two Scapharca populations verified that this PCR-generated technique could be a profitable attempt for within- and between-population-grounded biological DNA scrutiny. The potential of PCR inquiry will be favorable in the selection of individuals and/or populations for several reproductive- and/or quarantine-connected characters in aquafarming manufacture.

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