N Forin, A Vizzini, S Nigris, E Ercole, S Voyron, M Girlanda, B Baldan
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引用次数: 12
摘要
摘要利用MiSeq技术，从意大利沙卡多码头(Pier Andrea Saccardo)的真菌场中获得了nectriia spp.和rufofusca Nectriella的标本，并进行了形态学和分子生物学的研究。成功地从24个被鉴定为“nectriia”sensu Saccardo的标本(包括20个类型)和rufofusca的模式标本中获得了ITS1和ITS2序列。对于双歧黑蝇、弧度黑蝇和黑蝇，只恢复了ITS1序列。在形态学和分子生物学分析的基础上，建立了红木网蝗、双歧网蝗、双歧网蝗变种黄斑网蝗、细粒网蝗和紫斑网蝗的新命名组合。提出了reyesiana, radians, squamuligera, njibodensis和congesta, nflageoletiana, phyllostachydis, nsordescens和njibodensis var. crebris的新同义词。此外，冠状nectriia coronata、cyanostoma、N. dolichospora、N. illudens、N. leucotricha、N. mantuana、N. raripila和rufofusca的分类也得到了确认。这是第一次对这些100多年前的标本进行分子分析，从而为这些名字提供了重要的新的DNA序列数据。
Illuminating type collections of nectriaceous fungi in Saccardo's fungarium.
Specimens of Nectria spp. and Nectriella rufofusca were obtained from the fungarium of Pier Andrea Saccardo, and investigated via a morphological and molecular approach based on MiSeq technology. ITS1 and ITS2 sequences were successfully obtained from 24 specimens identified as 'Nectria' sensu Saccardo (including 20 types) and from the type specimen of Nectriella rufofusca. For Nectria ambigua, N. radians and N. tjibodensis only the ITS1 sequence was recovered. On the basis of morphological and molecular analyses new nomenclatural combinations for Nectria albofimbriata, N. ambigua, N. ambigua var. pallens, N. granuligera, N. peziza subsp. reyesiana, N. radians, N. squamuligera, N. tjibodensis and new synonymies for N. congesta, N. flageoletiana, N.phyllostachydis, N. sordescens and N. tjibodensis var. crebrior are proposed. Furthermore, the current classification is confirmed for Nectria coronata, N. cyanostoma, N. dolichospora, N. illudens, N. leucotricha, N. mantuana, N. raripila and Nectriella rufofusca. This is the first time that these more than 100-yr-old specimens are subjected to molecular analysis, thereby providing important new DNA sequence data authentic for these names.
期刊介绍：
Persoonia aspires to publish papers focusing on the molecular systematics and evolution of fungi. Additionally, it seeks to advance fungal taxonomy by employing a polythetic approach to elucidate the genuine phylogeny and relationships within the kingdom Fungi. The journal is dedicated to disseminating high-quality papers that unravel both known and novel fungal taxa at the DNA level. Moreover, it endeavors to provide fresh insights into evolutionary processes and relationships. The scope of papers considered encompasses research articles, along with topical and book reviews.
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