{"title":"PDLLA/β-TCP/HA/CHS/NGF对周围神经再生的缓释导管。","authors":"Xiumei Yan, Jing Wang, Qundi He, Haixing Xu, Junyan Tao, Kelly Koral, Kebi Li, Jingyi Xu, Jing Wen, Zhijun Huang, Peihu Xu","doi":"10.1007/s11595-021-2450-6","DOIUrl":null,"url":null,"abstract":"<p><p>Using nerve guide conduits (NGCs) to promote the regeneration of PNI is a feasible alternative to autograft. Compared with NGCs made of single material, composite NGCs have a greater development prospect. Our previous research has confirmed that poly(D, L-lactic acid)/<i>β</i>-tricalcium phosphate/hyaluronic acid/chitosan/nerve growth factor (PDLLA/<i>β</i>-TCP/HA/CHS/NGF) NGCs have excellent physical and chemical properties, which can slowly release NGF and support cell adhesion and proliferation. In this study, PDLLA/<i>β</i>-TCP/HA/CHS/NGF NGCs were prepared and used to bridge a 10 mm sciatic nerve defect in 200-250 g Sprague-Dawley (SD) rat to verify the performance of the NGCs <i>in vivo</i>. Substantial improvements in nerve regeneration were observed after using the PDLLA/<i>β</i>-TCP/HA/CHS/NGF NGCs based on gross post-operation observation, triceps wet weight analysis and nerve histological assessment. <i>In vivo</i> studies illustrate that the PDLLA/<i>β</i>-TCP/HA/CHS/NGF sustained-release NGCs can effectively promote peripheral nerve regeneration, and the effect is similar to that of autograft.</p>","PeriodicalId":49949,"journal":{"name":"Journal of Wuhan University of Technology-Materials Science Edition","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403253/pdf/","citationCount":"2","resultStr":"{\"title\":\"PDLLA/<i>β</i>-TCP/HA/CHS/NGF Sustained-release Conduits for Peripheral Nerve Regeneration.\",\"authors\":\"Xiumei Yan, Jing Wang, Qundi He, Haixing Xu, Junyan Tao, Kelly Koral, Kebi Li, Jingyi Xu, Jing Wen, Zhijun Huang, Peihu Xu\",\"doi\":\"10.1007/s11595-021-2450-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Using nerve guide conduits (NGCs) to promote the regeneration of PNI is a feasible alternative to autograft. Compared with NGCs made of single material, composite NGCs have a greater development prospect. Our previous research has confirmed that poly(D, L-lactic acid)/<i>β</i>-tricalcium phosphate/hyaluronic acid/chitosan/nerve growth factor (PDLLA/<i>β</i>-TCP/HA/CHS/NGF) NGCs have excellent physical and chemical properties, which can slowly release NGF and support cell adhesion and proliferation. In this study, PDLLA/<i>β</i>-TCP/HA/CHS/NGF NGCs were prepared and used to bridge a 10 mm sciatic nerve defect in 200-250 g Sprague-Dawley (SD) rat to verify the performance of the NGCs <i>in vivo</i>. Substantial improvements in nerve regeneration were observed after using the PDLLA/<i>β</i>-TCP/HA/CHS/NGF NGCs based on gross post-operation observation, triceps wet weight analysis and nerve histological assessment. <i>In vivo</i> studies illustrate that the PDLLA/<i>β</i>-TCP/HA/CHS/NGF sustained-release NGCs can effectively promote peripheral nerve regeneration, and the effect is similar to that of autograft.</p>\",\"PeriodicalId\":49949,\"journal\":{\"name\":\"Journal of Wuhan University of Technology-Materials Science Edition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403253/pdf/\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Wuhan University of Technology-Materials Science Edition\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.1007/s11595-021-2450-6\",\"RegionNum\":4,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/8/29 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Wuhan University of Technology-Materials Science Edition","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1007/s11595-021-2450-6","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/8/29 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 2
摘要
利用神经引导导管(NGCs)促进PNI再生是一种可行的替代自体移植物的方法。与单一材料制成的NGCs相比，复合NGCs具有更大的发展前景。我们前期研究证实，聚(D, l -乳酸)/β-磷酸三钙/透明质酸/壳聚糖/神经生长因子(PDLLA/β-TCP/HA/CHS/NGF) NGCs具有优异的理化性能，能缓慢释放NGF，支持细胞粘附和增殖。本研究制备了PDLLA/β-TCP/HA/CHS/NGF NGCs，并将其用于200-250 g SD大鼠10 mm坐骨神经缺损的桥接，验证其在体内的性能。术后大体观察、三头肌湿重分析和神经组织学评估显示，使用PDLLA/β-TCP/HA/CHS/NGF NGCs后，神经再生明显改善。体内研究表明，PDLLA/β-TCP/HA/CHS/NGF缓释NGCs能有效促进周围神经再生，其作用与自体移植物相似。
PDLLA/β-TCP/HA/CHS/NGF Sustained-release Conduits for Peripheral Nerve Regeneration.
Using nerve guide conduits (NGCs) to promote the regeneration of PNI is a feasible alternative to autograft. Compared with NGCs made of single material, composite NGCs have a greater development prospect. Our previous research has confirmed that poly(D, L-lactic acid)/β-tricalcium phosphate/hyaluronic acid/chitosan/nerve growth factor (PDLLA/β-TCP/HA/CHS/NGF) NGCs have excellent physical and chemical properties, which can slowly release NGF and support cell adhesion and proliferation. In this study, PDLLA/β-TCP/HA/CHS/NGF NGCs were prepared and used to bridge a 10 mm sciatic nerve defect in 200-250 g Sprague-Dawley (SD) rat to verify the performance of the NGCs in vivo. Substantial improvements in nerve regeneration were observed after using the PDLLA/β-TCP/HA/CHS/NGF NGCs based on gross post-operation observation, triceps wet weight analysis and nerve histological assessment. In vivo studies illustrate that the PDLLA/β-TCP/HA/CHS/NGF sustained-release NGCs can effectively promote peripheral nerve regeneration, and the effect is similar to that of autograft.
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The Journal publishes reviews, full-length papers, and short communications presenting original research in materials science and engineering, focusing on functional and structural materials, advanced technology for materials synthesis and processing, nanomaterials, composites, computational material science, etc. All papers published are subjected to peer review, and are edited to conform to related international standards.
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