具有抗菌和成骨双重功能的肽基逐层涂层

IF 8.1 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Materials science & engineering. C, Materials for biological applications Pub Date : 2021-12-01 DOI:10.1016/j.msec.2021.112479
Charlotte Roupie , Béatrice Labat , Sandrine Morin-Grognet , Aline Echalard , Guy Ladam , Pascal Thébault
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引用次数: 8

摘要

植入式生物材料是骨外科领域的基石。它们的表面有望满足两个特殊的要求:防止细菌的沉降和发展,以及刺激骨细胞以促进骨整合。因此,现代方法包括设计具有抗菌和成骨功能的双重功能涂层。为此,我们开发了由生物相容性聚电解质,即硫酸软骨素A (CSA)和聚赖氨酸(PLL)组成的超薄层逐层(LbL)涂层。涂层与天然的生物相容性交联剂genipin (GnP)交联,以增加其对环境变化的抵抗力,并赋予其与骨细胞行为相关的足够的机械性能。用抗菌肽(AMP) nisin Z对革兰氏阳性菌具有抑菌活性。该涂层对金黄色葡萄球菌具有显著的杀菌作用,能充分维持骨细胞的粘附、增殖和成骨分化。
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Dual-functional antibacterial and osteogenic nisin-based layer-by-layer coatings

Implanted biomaterials can be regarded in a cornerstone in the domain of bone surgery. Their surfaces are expected to fulfil two particular requirements: preventing the settlement and the development of bacteria, and stimulating bone cells in view to foster osseointegration. Therefore, a modern approach consists in the design of dual functional coatings with both antibacterial and osteogenic features. To this end, we developed ultrathin Layer-by-Layer (LbL) coatings composed of biocompatible polyelectrolytes, namely chondroitin sulfate A (CSA) and poly-l-lysine (PLL). The coatings were crosslinked with genipin (GnP), a natural and biocompatible crosslinking agent, to increase their resistance against environmental changes, and to confer them adequate mechanical properties with regards to bone cell behaviors. Antibacterial activity was obtained with nisin Z, an antimicrobial peptide (AMP), which is active against gram-positive bacteria. The coatings had a significant bactericidal impact upon Staphylococcus aureus, with fully maintained bone cell adhesion, proliferation and osteogenic differentiation.

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来源期刊
CiteScore
12.60
自引率
0.00%
发文量
28
审稿时长
3.3 months
期刊介绍: Materials Today is a community committed to fostering the creation and sharing of knowledge and experience in materials science. With the support of Elsevier, this community publishes high-impact peer-reviewed journals, organizes academic conferences, and conducts educational webinars, among other initiatives. It serves as a hub for advancing materials science and facilitating collaboration within the scientific community.
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