{"title":"微量营养素对人体硫化氢代谢的调节作用，初步数据。","authors":"Maurizio Dattilo, Carolina Fontanarosa, Michele Spinelli, Vittorio Bini, Angela Amoresano","doi":"10.1177/11786388211065372","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hydrogen sulfide (H<sub>2</sub>S) is a pivotal gasotransmitter networking with nitric oxide (NO) and carbon monoxide (CO) to regulate basic homeostatic functions. It is released by the alternative pathways of transulfuration by the enzymes Cystathionine Beta Synthase (CBS) and Cystathionine Gamma Lyase (CSE), and by Cysteine AminoTransferase (CAT)/ 3-Mercaptopyruvate Sulfur Transferase (3MPST). A non-enzymatic, intravascular release is also in place. We retrospectively investigated the possibility to modulate the endogenous H<sub>2</sub>S release and signaling in humans by a dietary manipulation with supplemented micronutrients (L-cystine, Taurine and pyridoxal 5-phopsphate/P5P).</p><p><strong>Methods: </strong>Patients referring for antiaging purposes underwent a 10-day supplementation. Blood was collected at baseline and after treatment and the metabolome was investigated by mass spectrometry to monitor the changes in the metabolites reporting on H<sub>2</sub>S metabolism and related pathways.</p><p><strong>Results: </strong>Data were available from 6 middle aged subjects (2 women). Micronutrients increased 3-mercaptopyruvate (<i>P</i> = .03), reporting on the activity of CAT that provides the substrate for H<sub>2</sub>S release within mitochondria by 3MPST, decreased lanthionine (<i>P</i> = .024), reporting the release of H<sub>2</sub>S from CBS, and had no significant effect of H<sub>2</sub>S release from CSE. This is compatible with a homeostatic balancing. We also recorded a strong increase of reporters of H<sub>2</sub>S-induced pathways including 5-MethylTHF (<i>P</i> = .001) and SAME (<i>P</i> = .022), reporting on methylation capacity, and of BH4 (<i>P</i> = .021) and BH2 (<i>P</i> = .028) reporting on nitric oxide metabolism. These activations may be explained by the concomitant induction of non-enzymatic release of H<sub>2</sub>S.</p><p><strong>Conclusions: </strong>Although the current evidences are weak and will need to be confirmed, the effect of micronutrients was compatible with an increase of the H<sub>2</sub>S endogenous release and signaling within the control of homeostatic mechanisms, further endorsing the role of feeding in health and disease. These effects might result in a H<sub>2</sub>S boosting effect in case of defective activity of pathologic origin, which should be checked in duly designed clinical trials.</p>","PeriodicalId":19396,"journal":{"name":"Nutrition and Metabolic Insights","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2022-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/71/dd/10.1177_11786388211065372.PMC8743967.pdf","citationCount":"0","resultStr":"{\"title\":\"Modulation of Human Hydrogen Sulfide Metabolism by Micronutrients, Preliminary Data.\",\"authors\":\"Maurizio Dattilo, Carolina Fontanarosa, Michele Spinelli, Vittorio Bini, Angela Amoresano\",\"doi\":\"10.1177/11786388211065372\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Hydrogen sulfide (H<sub>2</sub>S) is a pivotal gasotransmitter networking with nitric oxide (NO) and carbon monoxide (CO) to regulate basic homeostatic functions. It is released by the alternative pathways of transulfuration by the enzymes Cystathionine Beta Synthase (CBS) and Cystathionine Gamma Lyase (CSE), and by Cysteine AminoTransferase (CAT)/ 3-Mercaptopyruvate Sulfur Transferase (3MPST). A non-enzymatic, intravascular release is also in place. We retrospectively investigated the possibility to modulate the endogenous H<sub>2</sub>S release and signaling in humans by a dietary manipulation with supplemented micronutrients (L-cystine, Taurine and pyridoxal 5-phopsphate/P5P).</p><p><strong>Methods: </strong>Patients referring for antiaging purposes underwent a 10-day supplementation. Blood was collected at baseline and after treatment and the metabolome was investigated by mass spectrometry to monitor the changes in the metabolites reporting on H<sub>2</sub>S metabolism and related pathways.</p><p><strong>Results: </strong>Data were available from 6 middle aged subjects (2 women). Micronutrients increased 3-mercaptopyruvate (<i>P</i> = .03), reporting on the activity of CAT that provides the substrate for H<sub>2</sub>S release within mitochondria by 3MPST, decreased lanthionine (<i>P</i> = .024), reporting the release of H<sub>2</sub>S from CBS, and had no significant effect of H<sub>2</sub>S release from CSE. This is compatible with a homeostatic balancing. We also recorded a strong increase of reporters of H<sub>2</sub>S-induced pathways including 5-MethylTHF (<i>P</i> = .001) and SAME (<i>P</i> = .022), reporting on methylation capacity, and of BH4 (<i>P</i> = .021) and BH2 (<i>P</i> = .028) reporting on nitric oxide metabolism. These activations may be explained by the concomitant induction of non-enzymatic release of H<sub>2</sub>S.</p><p><strong>Conclusions: </strong>Although the current evidences are weak and will need to be confirmed, the effect of micronutrients was compatible with an increase of the H<sub>2</sub>S endogenous release and signaling within the control of homeostatic mechanisms, further endorsing the role of feeding in health and disease. These effects might result in a H<sub>2</sub>S boosting effect in case of defective activity of pathologic origin, which should be checked in duly designed clinical trials.</p>\",\"PeriodicalId\":19396,\"journal\":{\"name\":\"Nutrition and Metabolic Insights\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-01-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/71/dd/10.1177_11786388211065372.PMC8743967.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nutrition and Metabolic Insights\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/11786388211065372\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nutrition and Metabolic Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/11786388211065372","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Modulation of Human Hydrogen Sulfide Metabolism by Micronutrients, Preliminary Data.
Background: Hydrogen sulfide (H2S) is a pivotal gasotransmitter networking with nitric oxide (NO) and carbon monoxide (CO) to regulate basic homeostatic functions. It is released by the alternative pathways of transulfuration by the enzymes Cystathionine Beta Synthase (CBS) and Cystathionine Gamma Lyase (CSE), and by Cysteine AminoTransferase (CAT)/ 3-Mercaptopyruvate Sulfur Transferase (3MPST). A non-enzymatic, intravascular release is also in place. We retrospectively investigated the possibility to modulate the endogenous H2S release and signaling in humans by a dietary manipulation with supplemented micronutrients (L-cystine, Taurine and pyridoxal 5-phopsphate/P5P).
Methods: Patients referring for antiaging purposes underwent a 10-day supplementation. Blood was collected at baseline and after treatment and the metabolome was investigated by mass spectrometry to monitor the changes in the metabolites reporting on H2S metabolism and related pathways.
Results: Data were available from 6 middle aged subjects (2 women). Micronutrients increased 3-mercaptopyruvate (P = .03), reporting on the activity of CAT that provides the substrate for H2S release within mitochondria by 3MPST, decreased lanthionine (P = .024), reporting the release of H2S from CBS, and had no significant effect of H2S release from CSE. This is compatible with a homeostatic balancing. We also recorded a strong increase of reporters of H2S-induced pathways including 5-MethylTHF (P = .001) and SAME (P = .022), reporting on methylation capacity, and of BH4 (P = .021) and BH2 (P = .028) reporting on nitric oxide metabolism. These activations may be explained by the concomitant induction of non-enzymatic release of H2S.
Conclusions: Although the current evidences are weak and will need to be confirmed, the effect of micronutrients was compatible with an increase of the H2S endogenous release and signaling within the control of homeostatic mechanisms, further endorsing the role of feeding in health and disease. These effects might result in a H2S boosting effect in case of defective activity of pathologic origin, which should be checked in duly designed clinical trials.
期刊介绍：
Nutrition and Metabolic Insights is a peer-reviewed, open-access online journal focusing on all aspects of nutrition and metabolism. This encompasses nutrition, including the biochemistry of metabolism, exercise and associated physical processes and also includes clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes. It includes research at the molecular, cellular and organismal levels. This journal welcomes new manuscripts for peer review on the following topics: Nutrition, including the biochemistry of metabolism, Exercise and associated physical processes, Clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes, Research at the molecular, cellular and organismal levels, Other areas of interest include gene-nutrient interactions, the effects of hormones, models of metabolic function, macronutrient interactions, outcomes of changes in diet, and pathophysiology.
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