{"title":"微量营养素对人体硫化氢代谢的调节作用，初步数据。","authors":"Maurizio Dattilo, Carolina Fontanarosa, Michele Spinelli, Vittorio Bini, Angela Amoresano","doi":"10.1177/11786388211065372","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hydrogen sulfide (H<sub>2</sub>S) is a pivotal gasotransmitter networking with nitric oxide (NO) and carbon monoxide (CO) to regulate basic homeostatic functions. It is released by the alternative pathways of transulfuration by the enzymes Cystathionine Beta Synthase (CBS) and Cystathionine Gamma Lyase (CSE), and by Cysteine AminoTransferase (CAT)/ 3-Mercaptopyruvate Sulfur Transferase (3MPST). A non-enzymatic, intravascular release is also in place. We retrospectively investigated the possibility to modulate the endogenous H<sub>2</sub>S release and signaling in humans by a dietary manipulation with supplemented micronutrients (L-cystine, Taurine and pyridoxal 5-phopsphate/P5P).</p><p><strong>Methods: </strong>Patients referring for antiaging purposes underwent a 10-day supplementation. Blood was collected at baseline and after treatment and the metabolome was investigated by mass spectrometry to monitor the changes in the metabolites reporting on H<sub>2</sub>S metabolism and related pathways.</p><p><strong>Results: </strong>Data were available from 6 middle aged subjects (2 women). Micronutrients increased 3-mercaptopyruvate (<i>P</i> = .03), reporting on the activity of CAT that provides the substrate for H<sub>2</sub>S release within mitochondria by 3MPST, decreased lanthionine (<i>P</i> = .024), reporting the release of H<sub>2</sub>S from CBS, and had no significant effect of H<sub>2</sub>S release from CSE. This is compatible with a homeostatic balancing. We also recorded a strong increase of reporters of H<sub>2</sub>S-induced pathways including 5-MethylTHF (<i>P</i> = .001) and SAME (<i>P</i> = .022), reporting on methylation capacity, and of BH4 (<i>P</i> = .021) and BH2 (<i>P</i> = .028) reporting on nitric oxide metabolism. These activations may be explained by the concomitant induction of non-enzymatic release of H<sub>2</sub>S.</p><p><strong>Conclusions: </strong>Although the current evidences are weak and will need to be confirmed, the effect of micronutrients was compatible with an increase of the H<sub>2</sub>S endogenous release and signaling within the control of homeostatic mechanisms, further endorsing the role of feeding in health and disease. These effects might result in a H<sub>2</sub>S boosting effect in case of defective activity of pathologic origin, which should be checked in duly designed clinical trials.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"11786388211065372"},"PeriodicalIF":4.7000,"publicationDate":"2022-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/71/dd/10.1177_11786388211065372.PMC8743967.pdf","citationCount":"0","resultStr":"{\"title\":\"Modulation of Human Hydrogen Sulfide Metabolism by Micronutrients, Preliminary Data.\",\"authors\":\"Maurizio Dattilo, Carolina Fontanarosa, Michele Spinelli, Vittorio Bini, Angela Amoresano\",\"doi\":\"10.1177/11786388211065372\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Hydrogen sulfide (H<sub>2</sub>S) is a pivotal gasotransmitter networking with nitric oxide (NO) and carbon monoxide (CO) to regulate basic homeostatic functions. It is released by the alternative pathways of transulfuration by the enzymes Cystathionine Beta Synthase (CBS) and Cystathionine Gamma Lyase (CSE), and by Cysteine AminoTransferase (CAT)/ 3-Mercaptopyruvate Sulfur Transferase (3MPST). A non-enzymatic, intravascular release is also in place. We retrospectively investigated the possibility to modulate the endogenous H<sub>2</sub>S release and signaling in humans by a dietary manipulation with supplemented micronutrients (L-cystine, Taurine and pyridoxal 5-phopsphate/P5P).</p><p><strong>Methods: </strong>Patients referring for antiaging purposes underwent a 10-day supplementation. Blood was collected at baseline and after treatment and the metabolome was investigated by mass spectrometry to monitor the changes in the metabolites reporting on H<sub>2</sub>S metabolism and related pathways.</p><p><strong>Results: </strong>Data were available from 6 middle aged subjects (2 women). Micronutrients increased 3-mercaptopyruvate (<i>P</i> = .03), reporting on the activity of CAT that provides the substrate for H<sub>2</sub>S release within mitochondria by 3MPST, decreased lanthionine (<i>P</i> = .024), reporting the release of H<sub>2</sub>S from CBS, and had no significant effect of H<sub>2</sub>S release from CSE. This is compatible with a homeostatic balancing. We also recorded a strong increase of reporters of H<sub>2</sub>S-induced pathways including 5-MethylTHF (<i>P</i> = .001) and SAME (<i>P</i> = .022), reporting on methylation capacity, and of BH4 (<i>P</i> = .021) and BH2 (<i>P</i> = .028) reporting on nitric oxide metabolism. These activations may be explained by the concomitant induction of non-enzymatic release of H<sub>2</sub>S.</p><p><strong>Conclusions: </strong>Although the current evidences are weak and will need to be confirmed, the effect of micronutrients was compatible with an increase of the H<sub>2</sub>S endogenous release and signaling within the control of homeostatic mechanisms, further endorsing the role of feeding in health and disease. These effects might result in a H<sub>2</sub>S boosting effect in case of defective activity of pathologic origin, which should be checked in duly designed clinical trials.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":\" \",\"pages\":\"11786388211065372\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2022-01-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/71/dd/10.1177_11786388211065372.PMC8743967.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/11786388211065372\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/11786388211065372","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Modulation of Human Hydrogen Sulfide Metabolism by Micronutrients, Preliminary Data.
Background: Hydrogen sulfide (H2S) is a pivotal gasotransmitter networking with nitric oxide (NO) and carbon monoxide (CO) to regulate basic homeostatic functions. It is released by the alternative pathways of transulfuration by the enzymes Cystathionine Beta Synthase (CBS) and Cystathionine Gamma Lyase (CSE), and by Cysteine AminoTransferase (CAT)/ 3-Mercaptopyruvate Sulfur Transferase (3MPST). A non-enzymatic, intravascular release is also in place. We retrospectively investigated the possibility to modulate the endogenous H2S release and signaling in humans by a dietary manipulation with supplemented micronutrients (L-cystine, Taurine and pyridoxal 5-phopsphate/P5P).
Methods: Patients referring for antiaging purposes underwent a 10-day supplementation. Blood was collected at baseline and after treatment and the metabolome was investigated by mass spectrometry to monitor the changes in the metabolites reporting on H2S metabolism and related pathways.
Results: Data were available from 6 middle aged subjects (2 women). Micronutrients increased 3-mercaptopyruvate (P = .03), reporting on the activity of CAT that provides the substrate for H2S release within mitochondria by 3MPST, decreased lanthionine (P = .024), reporting the release of H2S from CBS, and had no significant effect of H2S release from CSE. This is compatible with a homeostatic balancing. We also recorded a strong increase of reporters of H2S-induced pathways including 5-MethylTHF (P = .001) and SAME (P = .022), reporting on methylation capacity, and of BH4 (P = .021) and BH2 (P = .028) reporting on nitric oxide metabolism. These activations may be explained by the concomitant induction of non-enzymatic release of H2S.
Conclusions: Although the current evidences are weak and will need to be confirmed, the effect of micronutrients was compatible with an increase of the H2S endogenous release and signaling within the control of homeostatic mechanisms, further endorsing the role of feeding in health and disease. These effects might result in a H2S boosting effect in case of defective activity of pathologic origin, which should be checked in duly designed clinical trials.
期刊介绍：
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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