甘草浆制备的水参水对体外葡萄糖性白内障的预防作用。

Ayu Pub Date : 2020-04-01 Epub Date: 2021-10-23 DOI:10.4103/ayu.AYU_99_20
Manjusha Rajagopala, B Ravishankar, B K Ashok, B Gupta Varun
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摘要

背景:白内障是全世界失明的主要原因。传统医学如阿育吠陀,除了通过手术摘除白内障外,还通过抗氧化活性来解决白内障的问题,受到了研究人员的广泛关注。目的:研究在家禽(Gallus Gallus)的Vasa(脂肪)中加入Yashtimadhu (Glycyrrhyza glabra Linn) Kalka(膏体)制备的Vasanjana (VK)对羊(羊)晶状体葡萄糖性白内障的抗白内障活性。材料和方法:采用含55 mM葡萄糖的人工房水诱导羊眼晶状体白内障。将牛油(CG)、普通脂肪、Vasanjana和维生素E添加到相同的培养基中,在室温下培养72 h。研究晶状体的生化参数为总蛋白、丙二醛(MDA)、Na+ K+ atp酶活性和电解质(Na+和K+)。还进行了摄影评价。结果:葡萄糖作用72 h使羊晶状体完全混浊,晶状体内Na+、MDA水平显著升高,Na+ K+ atp酶活性和总蛋白含量显著降低。照片评价表明,Vasanjana处理晶状体后,总蛋白含量无明显增加,MDA水平降低,葡萄糖抑制白内障的形成和进展。葡萄糖诱导的生化变化在CG和维生素E处理的晶状体中有统计学意义的逆转。结论:黄芪和黄芪具有抗白内障作用可能与黄芪的抗氧化和自由基清除作用有关。进一步的体外和体内研究需要在各种实验模型中验证其抗白内障活性。
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Prevention of in vitro glucose-induced cataract by Vasanjana prepared by Yashtimadhu Kalka (paste of Glycyrrhyza glabra Linn).

Background: Cataract is a major cause of blindness worldwide. Researchers received much attention from the traditional systems such as Ayurveda for the solutions of cataract through antioxidant activities apart from the surgical extraction.

Aims: A To study, the anti-cataract activity of Vasanjana (VK) prepared with Yashtimadhu (Glycyrrhyza glabra Linn) Kalka (paste) in Vasa (fat) of the domestic fowl (Gallus gallus) on glucose-induced cataract in ovine (sheep) lenses.

Materials and methods: Artificial aqueous humor with 55 mM glucose was used to induce cataract in sheep eye lenses. Treatment was given with cow ghee (CG), plain fat, Vasanjana, and Vitamin E to the same media and lenses were incubated at the room temperature for 72 h. Biochemical parameters studied in the lens were total proteins, malondialdehyde (MDA), Na+ K+ ATPase activity and electrolytes (Na+ and K+). Photographic evaluation was also done.

Results: The complete opacification induced by the glucose in ovine lens was observed in 72 h. Cataractous lenses showed significant increase in Na+, MDA level and significant decrease in Na+ K+ ATPase activity and total protein content. Lenses treated with Vasanjana showed non-significant increase of total protein content and decreased MDA level and prevented formation and progress of cataract by glucose, as evidenced by photographic evaluation. Glucose-induced biochemical changes were found to be reversed in statistically significant manner in CG and Vitamin E treated lenses.

Conclusion: The anti-cataract activity of Vasanjana and CG may be because of the antioxidant and free radical scavenging activity. Further in vitro and in vivo studies in various experimental models are required to validate their anti-cataract activity.

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