{"title":"紫花苜蓿皂苷通过 MAPK 信号通路抑制氧化应激诱导的细胞凋亡。","authors":"Yalei Cui, Fen Li, Xiaoyan Zhu, Junying Xu, Abaidullah Muhammad, Yanyan Chen, Defeng Li, Boshuai Liu, Chengzhang Wang, Zhichang Wang, Sen Ma, Xule Liu, Yinghua Shi","doi":"10.1080/13510002.2021.2017681","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Oxidative stress could seriously affect the growth performance of piglets. As natural extracts of <i>Alfalfa (Medicago sativa)</i>, alfalfa saponins have been shown to function as antioxidants in piglets <i>in vivo</i>. However, few studies have investigated the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells <i>in vitro</i>. In the current study, piglets' small intestinal epithelial cell line (IPEC-J2) was explored to investigate the protective effects of alfalfa saponins on injured cells induced by H<sub>2</sub>O<sub>2</sub>.</p><p><strong>Methods: </strong>To investigate the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells, the cell viability, activity of antioxidant enzymes, LDH and the amount of MDA were detected in H<sub>2</sub>O<sub>2</sub>-treated cells after the cells were pre-incubated with alfalfa saponins. The mechanism of alfalfa saponins against H<sub>2</sub>O<sub>2</sub>-induced oxidative cell damage was explored by detecting the expression of mitochondrial apoptosis-related proteins. Furthermore, the signaling pathway of alfalfa saponins in IPEC-J2 cells under oxidative stress was also investigated.</p><p><strong>Results: </strong>The results indicated that alfalfa saponins could rescue cell viability, elevate the activity of antioxidant enzymes and down-regulate the activity of LDH and the amount of MDA in H<sub>2</sub>O<sub>2</sub>-induced cells.</p><p><strong>Conclusion: </strong>Alfalfa saponins could inhibit oxidative stress-induced cell mitochondrial apoptosis through the MAPK signaling pathway, thereby providing a new method for improving antioxidant stress ability by means of nutritional regulation.</p>","PeriodicalId":5,"journal":{"name":"ACS Applied Materials & Interfaces","volume":" ","pages":"1-8"},"PeriodicalIF":8.2000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725750/pdf/","citationCount":"0","resultStr":"{\"title\":\"Alfalfa saponins inhibit oxidative stress-induced cell apoptosis through the MAPK signaling pathway.\",\"authors\":\"Yalei Cui, Fen Li, Xiaoyan Zhu, Junying Xu, Abaidullah Muhammad, Yanyan Chen, Defeng Li, Boshuai Liu, Chengzhang Wang, Zhichang Wang, Sen Ma, Xule Liu, Yinghua Shi\",\"doi\":\"10.1080/13510002.2021.2017681\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Oxidative stress could seriously affect the growth performance of piglets. As natural extracts of <i>Alfalfa (Medicago sativa)</i>, alfalfa saponins have been shown to function as antioxidants in piglets <i>in vivo</i>. However, few studies have investigated the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells <i>in vitro</i>. In the current study, piglets' small intestinal epithelial cell line (IPEC-J2) was explored to investigate the protective effects of alfalfa saponins on injured cells induced by H<sub>2</sub>O<sub>2</sub>.</p><p><strong>Methods: </strong>To investigate the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells, the cell viability, activity of antioxidant enzymes, LDH and the amount of MDA were detected in H<sub>2</sub>O<sub>2</sub>-treated cells after the cells were pre-incubated with alfalfa saponins. The mechanism of alfalfa saponins against H<sub>2</sub>O<sub>2</sub>-induced oxidative cell damage was explored by detecting the expression of mitochondrial apoptosis-related proteins. Furthermore, the signaling pathway of alfalfa saponins in IPEC-J2 cells under oxidative stress was also investigated.</p><p><strong>Results: </strong>The results indicated that alfalfa saponins could rescue cell viability, elevate the activity of antioxidant enzymes and down-regulate the activity of LDH and the amount of MDA in H<sub>2</sub>O<sub>2</sub>-induced cells.</p><p><strong>Conclusion: </strong>Alfalfa saponins could inhibit oxidative stress-induced cell mitochondrial apoptosis through the MAPK signaling pathway, thereby providing a new method for improving antioxidant stress ability by means of nutritional regulation.</p>\",\"PeriodicalId\":5,\"journal\":{\"name\":\"ACS Applied Materials & Interfaces\",\"volume\":\" \",\"pages\":\"1-8\"},\"PeriodicalIF\":8.2000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725750/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Materials & Interfaces\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/13510002.2021.2017681\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Materials & Interfaces","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/13510002.2021.2017681","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
Alfalfa saponins inhibit oxidative stress-induced cell apoptosis through the MAPK signaling pathway.
Background: Oxidative stress could seriously affect the growth performance of piglets. As natural extracts of Alfalfa (Medicago sativa), alfalfa saponins have been shown to function as antioxidants in piglets in vivo. However, few studies have investigated the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells in vitro. In the current study, piglets' small intestinal epithelial cell line (IPEC-J2) was explored to investigate the protective effects of alfalfa saponins on injured cells induced by H2O2.
Methods: To investigate the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells, the cell viability, activity of antioxidant enzymes, LDH and the amount of MDA were detected in H2O2-treated cells after the cells were pre-incubated with alfalfa saponins. The mechanism of alfalfa saponins against H2O2-induced oxidative cell damage was explored by detecting the expression of mitochondrial apoptosis-related proteins. Furthermore, the signaling pathway of alfalfa saponins in IPEC-J2 cells under oxidative stress was also investigated.
Results: The results indicated that alfalfa saponins could rescue cell viability, elevate the activity of antioxidant enzymes and down-regulate the activity of LDH and the amount of MDA in H2O2-induced cells.
Conclusion: Alfalfa saponins could inhibit oxidative stress-induced cell mitochondrial apoptosis through the MAPK signaling pathway, thereby providing a new method for improving antioxidant stress ability by means of nutritional regulation.
期刊介绍：
ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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