{"title":"-808纳米激活的Ca2+掺杂上转换纳米颗粒释放不诱导肝癌细胞(HepG2)凋亡。","authors":"Xinmeng Fa, Shaowei Lin, Jianghua Yang, Chong Shen, Yuanli Liu, Yongyang Gong, Aimiao Qin, Jun Ou, Ute Resch-Genger","doi":"10.1088/2050-6120/ac5524","DOIUrl":null,"url":null,"abstract":"<p><p>A near-infrared (NIR) light-triggered release method for nitric oxide (NO) was developed utilizing core/shell NaYF<sub>4</sub>: Tm/Yb/Ca@NaGdF<sub>4</sub>: Nd/Yb up-conversion nanoparticles (UCNPs) bearing a mesoporous silica (mSiO<sub>2</sub>) shell loaded with the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP). To avoid overheating in biological samples, Nd<sup>3+</sup>was chosen as a sensitizer, Yb<sup>3+</sup>ions as the bridging sensitizer, and Tm<sup>3+</sup>ions as UV-emissive activator while co-doping with Ca<sup>2+</sup>was done to enhance the luminescence of the activator Tm<sup>3+</sup>. NO release from SNAP was triggered by an NIR-UV up-conversion process, initiated by 808 nm light absorbed by the Nd<sup>3+</sup>ions. NO release was confirmed by the Griess method. Under 808 nm irradiation, the viability of the liver cancer cell line HepG2 significantly decreased with increasing UCNPs@mSiO<sub>2</sub>-SNAP concentration. For a UCNPs@mSiO<sub>2</sub>-SNAP concentration of 200<i>μ</i>g ml<sup>-1</sup>, the cell survival probability was 47%. These results demonstrate that UCNPs@mSiO<sub>2</sub>-SNAP can induce the release of apoptosis-inducing NO by NIR irradiation.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"10 2","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2022-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"-808 nm-activated Ca<sup>2+</sup>doped up-conversion nanoparticles that release no inducing liver cancer cell (HepG2) apoptosis.\",\"authors\":\"Xinmeng Fa, Shaowei Lin, Jianghua Yang, Chong Shen, Yuanli Liu, Yongyang Gong, Aimiao Qin, Jun Ou, Ute Resch-Genger\",\"doi\":\"10.1088/2050-6120/ac5524\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A near-infrared (NIR) light-triggered release method for nitric oxide (NO) was developed utilizing core/shell NaYF<sub>4</sub>: Tm/Yb/Ca@NaGdF<sub>4</sub>: Nd/Yb up-conversion nanoparticles (UCNPs) bearing a mesoporous silica (mSiO<sub>2</sub>) shell loaded with the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP). To avoid overheating in biological samples, Nd<sup>3+</sup>was chosen as a sensitizer, Yb<sup>3+</sup>ions as the bridging sensitizer, and Tm<sup>3+</sup>ions as UV-emissive activator while co-doping with Ca<sup>2+</sup>was done to enhance the luminescence of the activator Tm<sup>3+</sup>. NO release from SNAP was triggered by an NIR-UV up-conversion process, initiated by 808 nm light absorbed by the Nd<sup>3+</sup>ions. NO release was confirmed by the Griess method. Under 808 nm irradiation, the viability of the liver cancer cell line HepG2 significantly decreased with increasing UCNPs@mSiO<sub>2</sub>-SNAP concentration. For a UCNPs@mSiO<sub>2</sub>-SNAP concentration of 200<i>μ</i>g ml<sup>-1</sup>, the cell survival probability was 47%. These results demonstrate that UCNPs@mSiO<sub>2</sub>-SNAP can induce the release of apoptosis-inducing NO by NIR irradiation.</p>\",\"PeriodicalId\":18596,\"journal\":{\"name\":\"Methods and Applications in Fluorescence\",\"volume\":\"10 2\",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2022-02-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Methods and Applications in Fluorescence\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1088/2050-6120/ac5524\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and Applications in Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1088/2050-6120/ac5524","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
-808 nm-activated Ca2+doped up-conversion nanoparticles that release no inducing liver cancer cell (HepG2) apoptosis.
A near-infrared (NIR) light-triggered release method for nitric oxide (NO) was developed utilizing core/shell NaYF4: Tm/Yb/Ca@NaGdF4: Nd/Yb up-conversion nanoparticles (UCNPs) bearing a mesoporous silica (mSiO2) shell loaded with the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP). To avoid overheating in biological samples, Nd3+was chosen as a sensitizer, Yb3+ions as the bridging sensitizer, and Tm3+ions as UV-emissive activator while co-doping with Ca2+was done to enhance the luminescence of the activator Tm3+. NO release from SNAP was triggered by an NIR-UV up-conversion process, initiated by 808 nm light absorbed by the Nd3+ions. NO release was confirmed by the Griess method. Under 808 nm irradiation, the viability of the liver cancer cell line HepG2 significantly decreased with increasing UCNPs@mSiO2-SNAP concentration. For a UCNPs@mSiO2-SNAP concentration of 200μg ml-1, the cell survival probability was 47%. These results demonstrate that UCNPs@mSiO2-SNAP can induce the release of apoptosis-inducing NO by NIR irradiation.
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Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.
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