Marie Colombe Agahozo, Mieke van Bockstal, Pieter J Westenend, Christine Galant, Kathleen Lambein, Emily Reisenbichler, Renata Sinke, Serena Wong, Carolien H M van Deurzen
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Stromal changes were scored on hematoxylin/eosin-stained whole slides. P4HA2 expression in DCIS-associated stroma was assessed by whole slide immunohistochemistry. One hundred DCIS lesions were evaluated by seven pathologists to study the interobserver variability in the assessment of stromal changes and stromal P4HA2 expression. High P4HA2 expression in stromal fibroblasts was present in 14.1% of the patients. High P4HA2 expression was associated with the presence of periductal stromal changes (P = 0.004). The interobserver variability was similar for the assessment of stromal changes and the percentage of P4HA2-positive fibroblasts. 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引用次数: 1
摘要
乳腺导管原位癌(Ductal carcinoma in situ, DCIS)能够诱导间质改变,这可能反映了DCIS与其微环境之间的串扰。这些变化包含预后信息,尽管观察间间基质变化评分的可变性是中等的。因此,需要对dcis相关基质进行更可靠的评估。本研究的目的是表征参与胶原生物合成的P4HA2在DCIS中的表达,并评估与组织形态学相比,P4HA2表达是否能够更可靠地评估DCIS相关基质。本研究纳入了410例DCIS患者。苏木精/伊红染色全片对基质变化进行评分。采用全片免疫组织化学方法检测P4HA2在dcis相关基质中的表达。由7名病理学家对100个DCIS病变进行评估,以研究在评估间质变化和间质P4HA2表达方面的观察者间变异性。14.1%的患者间质成纤维细胞中P4HA2高表达。P4HA2高表达与管周间质改变相关(P = 0.004)。在评估基质变化和p4ha2阳性成纤维细胞百分比时,观察者间的变异性相似。尽管我们证明了成纤维细胞中P4HA2的高表达与基质形态学变化之间的显著关联,但P4HA2的表达似乎不太可能被用作dcis相关基质的组织病理学评估的替代方法。
Stromal Changes are Associated with High P4HA2 Expression in Ductal Carcinoma in Situ of the Breast.
Ductal carcinoma in situ (DCIS) of the breast is able to induce stromal changes, which likely reflect the crosstalk between DCIS and its microenvironment. These changes harbor prognostic information, although the interobserver variability of scoring stromal changes is moderate. A more robust evaluation of the DCIS-associated stroma is therefore needed. The aim of this study was to characterize P4HA2 expression, which is involved in collagen biosynthesis, in DCIS and to assess whether P4HA2 expression enables a more robust evaluation of the DCIS-associated stroma compared to histomorphology. This study included 410 patients with DCIS. Stromal changes were scored on hematoxylin/eosin-stained whole slides. P4HA2 expression in DCIS-associated stroma was assessed by whole slide immunohistochemistry. One hundred DCIS lesions were evaluated by seven pathologists to study the interobserver variability in the assessment of stromal changes and stromal P4HA2 expression. High P4HA2 expression in stromal fibroblasts was present in 14.1% of the patients. High P4HA2 expression was associated with the presence of periductal stromal changes (P = 0.004). The interobserver variability was similar for the assessment of stromal changes and the percentage of P4HA2-positive fibroblasts. Although we demonstrated a significant association between high P4HA2 expression in fibroblasts and the morphological presence of stromal changes, it seems unlikely that P4HA2 expression can be used as an alternative for the histopathological evaluation of the DCIS-associated stroma.
期刊介绍:
Journal of Mammary Gland Biology and Neoplasia is the leading Journal in the field of mammary gland biology that provides researchers within and outside the field of mammary gland biology with an integrated source of information pertaining to the development, function, and pathology of the mammary gland and its function.
Commencing in 2015, the Journal will begin receiving and publishing a combination of reviews and original, peer-reviewed research. The Journal covers all topics related to the field of mammary gland biology, including mammary development, breast cancer biology, lactation, and milk composition and quality. The environmental, endocrine, nutritional, and molecular factors regulating these processes is covered, including from a comparative biology perspective.