铝掺杂氧化锌和氮化镓纳米颗粒的蛋白质电晕形成。

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2022-01-01 DOI:10.1177/22808000221131881
Vladimir Ciobanu, Francesco Roncari, Giacomo Ceccone, Tudor Braniste, Jessica Ponti, Alessia Bogni, Giuditta Guerrini, Domenico Cassano, Pascal Colpo, Ion Tiginyanu
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引用次数: 2

摘要

考虑到纳米粒子的反应性和远程控制其性质的可能性,半导体纳米粒子与生物分子的相互作用引起了越来越多的研究人员的兴趣,给周围的生物环境带来了机械、热或电刺激。本文报道了在铝掺杂氧化锌和氮化镓纳米颗粒上形成蛋白质电晕的系统比较研究。选用牛血清白蛋白作为蛋白质模型。动态光散射、透射电子显微镜和x射线光电子能谱技术已经被用来证明蛋白质电晕的形成以及胶体悬浮液的稳定性,它也可以作为表面活性剂。Bradford法研究了蛋白质在NPs表面的吸附,发现蛋白质吸附在NPs表面可用位点上的数量依赖于蛋白质的数量,在ZnO的情况下,NPs与蛋白质的饱和比大于5:1,这与DLS结果相关。此外,蛋白质的动力学表现出相对较快的吸附在NPs表面上,约25 min后出现饱和曲线。然而,GaN NPs表面上吸附的蛋白质非常少,用DLS技术或差速离心沉降法无法观察到水动力尺寸的变化。圆二色性分析表明,BSA附着在NPs表面后,二级结构发生了剧烈的结构变化。ZnO纳米颗粒吸附蛋白冠,这不能保护它们免受溶解,因此,当浓度超过25µg/mL时,该材料被证明对人角化细胞(HaCaT)具有高毒性。相比之下,不吸附蛋白冠的GaN纳米颗粒在高达50µg/mL的浓度下对HaCaT细胞没有毒性迹象,与ZnO纳米颗粒相比,在培养基中表现出更低的离子泄漏浓度。
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Protein-corona formation on aluminum doped zinc oxide and gallium nitride nanoparticles.
The interaction of semiconductor nanoparticles with bio-molecules attracts increasing interest of researchers, considering the reactivity of nanoparticles and the possibility to control their properties remotely giving mechanical, thermal, or electrical stimulus to the surrounding bio-environment. This work reports on a systematic comparative study of the protein-corona formation on aluminum doped zinc oxide and gallium nitride nanoparticles. Bovine serum albumin was chosen as a protein model. Dynamic light scattering, transmission electron microscopy and X-ray photoelectron spectroscopy techniques have been used to demonstrate the formation of protein-corona as well as the stability of the colloidal suspension given by BSA, which also works as a surfactant. The protein adsorption on the NPs surface studied by Bradford Assay showed the dependence on the quantity of proteins adsorbed to the available sites on the NPs surface, thus the saturation was observed at ratio higher than 5:1 (NPs:Proteins) in case of ZnO, these correlating with DLS results. Moreover, the kinetics of the proteins showed a relatively fast adsorption on the NPs surface with a saturation curve after about 25 min. GaN NPs, however, showed a very small amount of proteins adsorbed on the surface, a change in the hydrodynamic size being not observable with DLS technique or differential centrifugal sedimentation. The Circular Dichroism analysis suggests a drastic structural change in the secondary structure of the BSA after attaching on the NPs surface. The ZnO nanoparticles adsorb a protein-corona, which does not protect them against dissolution, and in consequence, the material proved to be highly toxic for Human keratinocyte cell line (HaCaT) at concentration above 25 µg/mL. In contrast, the GaN nanoparticles which do not adsorb a protein-corona, show no toxicity signs for HaCaT cells at concentration as high as 50 µg/mL, exhibiting much lower concentration of ions leakage in the culture medium as compared to ZnO nanoparticles.
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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