Nathaniel J Ennis, Dhanasekaran Dharumadurai, Joseph L Sevigny, Ryan Wilmot, Sulaiman M Alnaimat, Julia G Bryce, W Kelley Thomas, Louis S Tisa
{"title":"从新英格兰的建筑石块和印度泰米尔纳德邦历史遗址中发现的印度石遗址中分离出的 11 个嗜地肤菌株的基因组序列草案。","authors":"Nathaniel J Ennis, Dhanasekaran Dharumadurai, Joseph L Sevigny, Ryan Wilmot, Sulaiman M Alnaimat, Julia G Bryce, W Kelley Thomas, Louis S Tisa","doi":"10.7150/jgen.76121","DOIUrl":null,"url":null,"abstract":"<p><p>Metagenomic analysis of stone microbiome from samples collected in New England, USA and Tamil Nadu, India identified numerous Actinobacteria including <i>Geodermatphilaceae</i>. A culture-dependent approach was performed as a companion study with this culture-independent metagenomic analysis of these stone samples and resulted in the isolation of eleven <i>Geodermatphilaceae</i> strains (2 <i>Geodermatophilus</i> and 9 <i>Blastococcus</i> strains). The genomes of the 11 <i>Geodermatphilaceae</i> strains were sequenced and analyzed. The genomes for the two <i>Geodermatophilus</i> isolates, DF1-2 and TF2-6, were 4.45 and 4.75 Mb, respectively, while the Blastococcus genomes ranged in size from 3.98 to 5.48 Mb. Phylogenetic analysis, digital DNA:DNA hybridization (dDDH), and comparisons of the average nucleotide identities (ANI) suggest the isolates represent novel <i>Geodermatophilus</i> and <i>Blastococcus</i> species. Functional analysis of the <i>Geodermatphilaceae</i> genomes provides insight on the stone microbiome niche.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":" ","pages":"69-77"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516006/pdf/","citationCount":"0","resultStr":"{\"title\":\"Draft Genomes Sequences of 11 <i>Geodermatophilaceae</i> Strains Isolated from Building Stones from New England and Indian Stone Ruins found at historic sites in Tamil Nadu, India.\",\"authors\":\"Nathaniel J Ennis, Dhanasekaran Dharumadurai, Joseph L Sevigny, Ryan Wilmot, Sulaiman M Alnaimat, Julia G Bryce, W Kelley Thomas, Louis S Tisa\",\"doi\":\"10.7150/jgen.76121\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Metagenomic analysis of stone microbiome from samples collected in New England, USA and Tamil Nadu, India identified numerous Actinobacteria including <i>Geodermatphilaceae</i>. A culture-dependent approach was performed as a companion study with this culture-independent metagenomic analysis of these stone samples and resulted in the isolation of eleven <i>Geodermatphilaceae</i> strains (2 <i>Geodermatophilus</i> and 9 <i>Blastococcus</i> strains). The genomes of the 11 <i>Geodermatphilaceae</i> strains were sequenced and analyzed. The genomes for the two <i>Geodermatophilus</i> isolates, DF1-2 and TF2-6, were 4.45 and 4.75 Mb, respectively, while the Blastococcus genomes ranged in size from 3.98 to 5.48 Mb. Phylogenetic analysis, digital DNA:DNA hybridization (dDDH), and comparisons of the average nucleotide identities (ANI) suggest the isolates represent novel <i>Geodermatophilus</i> and <i>Blastococcus</i> species. Functional analysis of the <i>Geodermatphilaceae</i> genomes provides insight on the stone microbiome niche.</p>\",\"PeriodicalId\":15834,\"journal\":{\"name\":\"Journal of Genomics\",\"volume\":\" \",\"pages\":\"69-77\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516006/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Genomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7150/jgen.76121\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Genomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7150/jgen.76121","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Draft Genomes Sequences of 11 Geodermatophilaceae Strains Isolated from Building Stones from New England and Indian Stone Ruins found at historic sites in Tamil Nadu, India.
Metagenomic analysis of stone microbiome from samples collected in New England, USA and Tamil Nadu, India identified numerous Actinobacteria including Geodermatphilaceae. A culture-dependent approach was performed as a companion study with this culture-independent metagenomic analysis of these stone samples and resulted in the isolation of eleven Geodermatphilaceae strains (2 Geodermatophilus and 9 Blastococcus strains). The genomes of the 11 Geodermatphilaceae strains were sequenced and analyzed. The genomes for the two Geodermatophilus isolates, DF1-2 and TF2-6, were 4.45 and 4.75 Mb, respectively, while the Blastococcus genomes ranged in size from 3.98 to 5.48 Mb. Phylogenetic analysis, digital DNA:DNA hybridization (dDDH), and comparisons of the average nucleotide identities (ANI) suggest the isolates represent novel Geodermatophilus and Blastococcus species. Functional analysis of the Geodermatphilaceae genomes provides insight on the stone microbiome niche.
期刊介绍：
Journal of Genomics publishes papers of high quality in all areas of gene, genetics, genomics, proteomics, metabolomics, DNA/RNA, computational biology, bioinformatics, and other relevant areas of research and application. Articles published by the journal are rigorously peer-reviewed. Types of articles include: Research paper, Short research communication, Review or mini-reviews, Commentary, Database, Software.
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