辣椒素负载胶束:制备、表征及MDA-MB-231乳腺癌细胞系体外细胞毒性评价。

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food Technology and Biotechnology Pub Date : 2022-09-01 DOI:10.17113/ftb.60.03.22.7405
Velmurugan Shanmugham, Ravi Subban
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引用次数: 2

摘要

研究背景:乳腺癌是最常见的癌症之一,仍然是全世界妇女发病和死亡的主要原因。在发达国家,乳腺癌作为一种多因素疾病是一个主要的健康问题,其发病率在低收入和中等收入国家不断上升。大量研究表明,类胡萝卜素等植物化学物质可抑制乳腺癌的生长并诱导细胞凋亡。我们最近通过将辣椒素包裹在一种新的非离子表面活性剂——黄芪皂苷元聚乙二醇琥珀酸盐中,提高了辣椒素在水中的溶解度。因此,本研究旨在通过四氮唑染料MTT法评价水溶性辣椒素胶束对MDA-MB-231细胞的体外细胞毒性。实验方法:本研究从甜红辣椒(Capsicum annuum)中提取了一种疏水性类胡萝卜素辣椒素(capsanthin)。以辣椒素提取物(cap)和薯蓣皂苷元聚乙二醇琥珀酸1000 (DPGS-1000)为原料,采用固体分散法制备了辣椒素负载的薯蓣皂苷元聚乙二醇琥珀酸1000 (cap-DPGS-1000)胶束。采用紫外可见光谱、高效液相色谱(HPLC)、傅里叶变换红外光谱(FTIR)、x射线衍射(XRD)、粒径分布、多分散性和扫描电镜(SEM)对辣椒素提取物和cap-DPGS-1000胶束进行了表征。研究了辣椒素提取物和cap-DPGS-1000胶束对人三阴性乳腺癌细胞株MDA-MB-231的影响,考察了胶束的细胞活力、增殖和细胞毒性。结果与结论:包封的cap-DPGS-1000胶束在水中的溶解度大大提高,增加了局部给药的范围,是治疗残余癌性肿瘤的更好的给药选择。包封的辣椒素在模拟肠液(pH=6.8)中有缓释作用。我们的研究提出了一种持续的药物递送系统,确保有效和控制释放到受影响的部位。表征数据显示,包封后辣椒素的结构和官能团没有变化。cap-DPGS-1000胶束对MDA-MB-231乳腺癌细胞的IC50值为(3.10±1.09)μg/mL,远低于辣椒素提取物的(81.1±1.5)μg/mL。辣椒素提取物和辣椒素负载胶束是诱导癌细胞凋亡和增加活性氧(ROS)的有希望的候选药物。新颖性和科学贡献:该结果首次证实了辣椒素和辣椒素负载胶束对MDA-MB-231细胞株的细胞毒性作用。甜红辣椒辣椒素对三阴性MDA-MB-231细胞株具有显著的细胞毒作用。
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Capsanthin-Loaded Micelles: Preparation, Characterization and in vitro Evaluation of Cytotoxicity Using MDA-MB-231 Breast Cancer Cell Line.

Research background: Breast cancer is one of the most common cancers and remains a major cause of morbidity and mortality among women worldwide. In developed countries, breast cancer as a multifactorial disease is a major health concern, and its incidence is constantly rising in low and middle-income countries. Numerous studies have demonstrated that phytochemicals such as carotenoids inhibit breast cancer growth and induce apoptosis. We recently enhanced the solubility of capsanthin in water by encapsulating it in diosgenin polyethylene glycol succinate, a novel non-ionic surfactant. Thus, this study aims to evaluate the cytotoxicity of water-soluble capsanthin-loaded micelles in MDA-MB-231 cells in vitro through tetrazolium dye MTT assay.

Experimental approach: In the current study, capsanthin, a hydrophobic carotenoid, is extracted from sweet red pepper (Capsicum annuum). Capsanthin-loaded diosgenin polyethylene glycol succinate 1000 (cap-DPGS-1000) micelles were prepared from capsanthin extract (cap) and diosgenin polyethylene glycol succinate 1000 (DPGS-1000) using the solid dispersion method. The capsanthin extract and cap-DPGS-1000 micelles were characterized by UV-visible spectroscopy, high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), particle size distribution, polydispersity, and scanning electron microscopy (SEM). The effects of capsanthin extract and cap-DPGS-1000 micelles on a human triple-negative breast cancer cell line (MDA-MB-231) were tested to check the cell viability, proliferation and cytotoxicity of the micelles.

Results and conclusions: The solubility of encapsulated cap-DPGS-1000 micelles in water is greatly enhanced and leads to an increased scope for localized drug delivery, a better delivery option for treating residual cancerous tumours. The encapsulated capsanthin showed a sustained release in simulated intestinal fluid (pH=6.8). Our research proposes a sustained drug delivery system that ensures effective and controlled release to the affected site. The characterization data revealed no change in the structure and functional groups in the encapsulated capsanthin. The IC50 value of the cap-DPGS-1000 micelles against MDA-MB-231 breast cancer cells was (3.10±1.09) μg/mL, which is much lower than of capsanthin extract ((81.1±1.5) μg/mL). Capsanthin extract and capsanthin-loaded micelles are promising drug candidates to induce apoptosis and increase reactive oxygen species (ROS) in cancer cells.

Novelty and scientific contribution: The result shows the cytotoxic effect of capsanthin and capsanthin-loaded micelles on MDA-MB-231 cell line for the first time. Capsanthin from sweet red pepper (Capsicum annuum) showed remarkable cytotoxic effect on the triple-negative MDA-MB-231 cell line.

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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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