{"title":"奥昔康类非甾体抗炎药增强农杆菌介导的植物瞬时转化。","authors":"Seung-Won Choi, Kie Kumaishi, Reiko Motohashi, Harumi Enoki, Wiluk Chacuttayapong, Tadashi Takamizo, Hiroaki Saika, Masaki Endo, Tetsuya Yamada, Aya Hirose, Nobuya Koizuka, Seisuke Kimura, Yaichi Kawakatsu, Hiroyuki Koga, Emi Ito, Ken Shirasu, Yasunori Ichihashi","doi":"10.5511/plantbiotechnology.22.0312a","DOIUrl":null,"url":null,"abstract":"<p><p><i>Agrobacterium</i>-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase the efficiency of <i>Agrobacterium</i>-mediated transient transformation. TNX treatment increased the transformation efficiency of <i>Agrobacterium</i>-mediated transformation of <i>Arabidopsis thaliana</i> mature leaves by agroinfiltration. The increase of efficiency by TNX treatment was not observed in <i>dde2/ein2/pad4/sid2</i> quadruple mutant, indicating that TNX inhibits the immune system mediated by jasmonic acid, ethylene, and salicylic acid against to <i>Agrobacterium</i>. We also found that TNX-treatment is applicable for the transient expression and subcellular localization analysis of fluorescent-tagged proteins in Arabidopsis leaf cells. In addition, we found that TNX increases the efficiency of <i>Agrobacterium</i>-mediated transient transformation of Jatropha. Given that treatment with oxicam compounds is a simple and cost effective method, our findings will provide a new option to overcome limitations associated with <i>Agrobacterium</i>-mediated transformation of various plant species.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592935/pdf/plantbiotechnology-39-3-22.0312a.pdf","citationCount":"0","resultStr":"{\"title\":\"Oxicam-type nonsteroidal anti-inflammatory drugs enhance <i>Agrobacterium</i>-mediated transient transformation in plants.\",\"authors\":\"Seung-Won Choi, Kie Kumaishi, Reiko Motohashi, Harumi Enoki, Wiluk Chacuttayapong, Tadashi Takamizo, Hiroaki Saika, Masaki Endo, Tetsuya Yamada, Aya Hirose, Nobuya Koizuka, Seisuke Kimura, Yaichi Kawakatsu, Hiroyuki Koga, Emi Ito, Ken Shirasu, Yasunori Ichihashi\",\"doi\":\"10.5511/plantbiotechnology.22.0312a\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Agrobacterium</i>-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase the efficiency of <i>Agrobacterium</i>-mediated transient transformation. TNX treatment increased the transformation efficiency of <i>Agrobacterium</i>-mediated transformation of <i>Arabidopsis thaliana</i> mature leaves by agroinfiltration. The increase of efficiency by TNX treatment was not observed in <i>dde2/ein2/pad4/sid2</i> quadruple mutant, indicating that TNX inhibits the immune system mediated by jasmonic acid, ethylene, and salicylic acid against to <i>Agrobacterium</i>. We also found that TNX-treatment is applicable for the transient expression and subcellular localization analysis of fluorescent-tagged proteins in Arabidopsis leaf cells. In addition, we found that TNX increases the efficiency of <i>Agrobacterium</i>-mediated transient transformation of Jatropha. Given that treatment with oxicam compounds is a simple and cost effective method, our findings will provide a new option to overcome limitations associated with <i>Agrobacterium</i>-mediated transformation of various plant species.</p>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2022-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592935/pdf/plantbiotechnology-39-3-22.0312a.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.5511/plantbiotechnology.22.0312a\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.5511/plantbiotechnology.22.0312a","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Oxicam-type nonsteroidal anti-inflammatory drugs enhance Agrobacterium-mediated transient transformation in plants.
Agrobacterium-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase the efficiency of Agrobacterium-mediated transient transformation. TNX treatment increased the transformation efficiency of Agrobacterium-mediated transformation of Arabidopsis thaliana mature leaves by agroinfiltration. The increase of efficiency by TNX treatment was not observed in dde2/ein2/pad4/sid2 quadruple mutant, indicating that TNX inhibits the immune system mediated by jasmonic acid, ethylene, and salicylic acid against to Agrobacterium. We also found that TNX-treatment is applicable for the transient expression and subcellular localization analysis of fluorescent-tagged proteins in Arabidopsis leaf cells. In addition, we found that TNX increases the efficiency of Agrobacterium-mediated transient transformation of Jatropha. Given that treatment with oxicam compounds is a simple and cost effective method, our findings will provide a new option to overcome limitations associated with Agrobacterium-mediated transformation of various plant species.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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