{"title":"交替蒿醇的一般毒性和遗传毒性:一项新的28天雄性Sprague-Dawley大鼠多终点评估。","authors":"Yeqiu Miao, Dongxia Wang, Yiyi Chen, Xia Zhu, Xinyao Tang, Jing Zhang, Lishi Zhang, Jinyao Chen","doi":"10.1007/s12550-022-00466-y","DOIUrl":null,"url":null,"abstract":"<p><p>Alternariol (AOH) is one of the toxins of Alternaria, and it has been widely detected in a variety of foods. It has been reported to be cytotoxic, dermally toxic, genotoxic, and potentially carcinogenic in vitro. However, in vivo toxicity data are lacking. This study used a novel in vivo 28-day multi-endpoint (Pig-a assay + micronucleus test + comet assay) genotoxicity evaluation system to evaluate the general toxicity and genotoxicity of AOH. A total of 42 male Sprague-Dawley rats were randomly distributed into three AOH-treated groups (5.51, 10.03, and 22.05 µg/kg bw), one AOH high-dose recovery group (AOH-HR, 22.05 µg/kg bw), one positive control group (N-ethyl-N-nitrosourea, 40 mg/kg bw), and two vehicle control groups (corn oil and PBS). Treatments were administered by oral gavage for 28 consecutive days. Histopathological lesions were observed in the liver, kidney, and spleen in all AOH-treated groups. No statistical difference was found in each genotoxicity index within 28 days in the AOH-treated groups compared with those in the corn oil group. On day 42, in the AOH-HR group, the rate of Pig-a mutant phenotype reticulocytes (RET<sup>CD59-</sup>) significantly increased. On day 56, both RET<sup>CD59-</sup> and the rate of Pig-a mutant phenotype erythrocytes (RBC<sup>CD59-</sup>) were significantly reduced. These findings indicated that AOH might cumulatively induce genetic mutations.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"General toxicity and genotoxicity of alternariol: a novel 28-day multi-endpoint assessment in male Sprague-Dawley rats.\",\"authors\":\"Yeqiu Miao, Dongxia Wang, Yiyi Chen, Xia Zhu, Xinyao Tang, Jing Zhang, Lishi Zhang, Jinyao Chen\",\"doi\":\"10.1007/s12550-022-00466-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Alternariol (AOH) is one of the toxins of Alternaria, and it has been widely detected in a variety of foods. It has been reported to be cytotoxic, dermally toxic, genotoxic, and potentially carcinogenic in vitro. However, in vivo toxicity data are lacking. This study used a novel in vivo 28-day multi-endpoint (Pig-a assay + micronucleus test + comet assay) genotoxicity evaluation system to evaluate the general toxicity and genotoxicity of AOH. A total of 42 male Sprague-Dawley rats were randomly distributed into three AOH-treated groups (5.51, 10.03, and 22.05 µg/kg bw), one AOH high-dose recovery group (AOH-HR, 22.05 µg/kg bw), one positive control group (N-ethyl-N-nitrosourea, 40 mg/kg bw), and two vehicle control groups (corn oil and PBS). Treatments were administered by oral gavage for 28 consecutive days. Histopathological lesions were observed in the liver, kidney, and spleen in all AOH-treated groups. No statistical difference was found in each genotoxicity index within 28 days in the AOH-treated groups compared with those in the corn oil group. On day 42, in the AOH-HR group, the rate of Pig-a mutant phenotype reticulocytes (RET<sup>CD59-</sup>) significantly increased. On day 56, both RET<sup>CD59-</sup> and the rate of Pig-a mutant phenotype erythrocytes (RBC<sup>CD59-</sup>) were significantly reduced. These findings indicated that AOH might cumulatively induce genetic mutations.</p>\",\"PeriodicalId\":19060,\"journal\":{\"name\":\"Mycotoxin Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycotoxin Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s12550-022-00466-y\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/8/1 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycotoxin Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12550-022-00466-y","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/8/1 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
General toxicity and genotoxicity of alternariol: a novel 28-day multi-endpoint assessment in male Sprague-Dawley rats.
Alternariol (AOH) is one of the toxins of Alternaria, and it has been widely detected in a variety of foods. It has been reported to be cytotoxic, dermally toxic, genotoxic, and potentially carcinogenic in vitro. However, in vivo toxicity data are lacking. This study used a novel in vivo 28-day multi-endpoint (Pig-a assay + micronucleus test + comet assay) genotoxicity evaluation system to evaluate the general toxicity and genotoxicity of AOH. A total of 42 male Sprague-Dawley rats were randomly distributed into three AOH-treated groups (5.51, 10.03, and 22.05 µg/kg bw), one AOH high-dose recovery group (AOH-HR, 22.05 µg/kg bw), one positive control group (N-ethyl-N-nitrosourea, 40 mg/kg bw), and two vehicle control groups (corn oil and PBS). Treatments were administered by oral gavage for 28 consecutive days. Histopathological lesions were observed in the liver, kidney, and spleen in all AOH-treated groups. No statistical difference was found in each genotoxicity index within 28 days in the AOH-treated groups compared with those in the corn oil group. On day 42, in the AOH-HR group, the rate of Pig-a mutant phenotype reticulocytes (RETCD59-) significantly increased. On day 56, both RETCD59- and the rate of Pig-a mutant phenotype erythrocytes (RBCCD59-) were significantly reduced. These findings indicated that AOH might cumulatively induce genetic mutations.
期刊介绍:
Mycotoxin Research, the official publication of the Society for Mycotoxin Research, is a peer-reviewed, scientific journal dealing with all aspects related to toxic fungal metabolites. The journal publishes original research articles and reviews in all areas dealing with mycotoxins. As an interdisciplinary platform, Mycotoxin Research welcomes submission of scientific contributions in the following research fields:
- Ecology and genetics of mycotoxin formation
- Mode of action of mycotoxins, metabolism and toxicology
- Agricultural production and mycotoxins
- Human and animal health aspects, including exposure studies and risk assessment
- Food and feed safety, including occurrence, prevention, regulatory aspects, and control of mycotoxins
- Environmental safety and technology-related aspects of mycotoxins
- Chemistry, synthesis and analysis.